1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CX3CL1
  5. Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His)

Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His)

Cat. No.: HY-P72685
COA Handling Instructions

Fractalkine/CX3CL1 protein, a chemokine, binds CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. It regulates immune response, inflammation, adhesion, chemotaxis, and leukocyte migration. It activates integrins via CX3CR1-dependent and -independent pathways, binding to site 1 with CX3CR1 and site 2 without CX3CR1. Soluble Fractalkine/CX3CL1 attracts T-cells and monocytes, not neutrophils. Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His) is the recombinant human-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His) is 315 a.a., with molecular weight of 50-90 kDa.

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  • Biological Activity

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Description

Fractalkine/CX3CL1 protein, a chemokine, binds CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. It regulates immune response, inflammation, adhesion, chemotaxis, and leukocyte migration. It activates integrins via CX3CR1-dependent and -independent pathways, binding to site 1 with CX3CR1 and site 2 without CX3CR1. Soluble Fractalkine/CX3CL1 attracts T-cells and monocytes, not neutrophils. Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His) is the recombinant human-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His) is 315 a.a., with molecular weight of 50-90 kDa.

Background

Fractalkine/CX3CL1 is a chemokine that serves as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. Its signaling through the CX3CR1-CX3CL1 pathway plays various roles in different tissue compartments, including immune response, inflammation, cell adhesion, and chemotaxis. It regulates leukocyte adhesion and migration at the endothelium and can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, it activates integrins by binding to the classical ligand-binding site (site 1), while in the absence of CX3CR1, it binds to a second site (site 2) in integrins that is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form of Fractalkine/CX3CL1 acts as a chemotactic factor for T-cells and monocytes but not neutrophils.

Biological Activity

Measured by its binding ability in a functional ELISA.

Species

Human

Source

HEK293

Tag

C-His

Accession

P78423 (Q25-R339)

Gene ID
Molecular Construction
N-term
Fractalkine (Q25-R339)
Accession # P78423
His
C-term
Synonyms
Fractalkine; C-X3-C motif chemokine 1; Neurotactin; CX3CL1; FKN; NTT; SCYD1
AA Sequence

QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATR

Molecular Weight

50-90 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2 or PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Fractalkine/CX3CL1 Protein, Human (315a.a, HEK293, His)
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HY-P72685
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