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Fractalkine/CX3CL1 Protein, Human (Biotinylated, HEK293, His, Avi)

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Fractalkine/CX3CL1 is a chemokine that acts as a ligand to CX3CR1 and the integrins ITGAV: ITGB3 and ITGA4: ITGB1. CX3CL1 promotes lung cancer cell migration and invasion through the Src/FAK signaling pathway. CX3CL1 plays an important role in immune response, inflammation, cell adhesion and chemotaxis. Fractalkine/CX3CL1 Protein, Human (Biotinylated, HEK293, His, Avi) is the recombinant human-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-Avi, C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Fractalkine/CX3CL1 Protein, Human (Biotinylated, HEK293, His, Avi) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Fractalkine/CX3CL1 is a chemokine that acts as a ligand to CX3CR1 and the integrins ITGAV: ITGB3 and ITGA4: ITGB1. CX3CL1 promotes lung cancer cell migration and invasion through the Src/FAK signaling pathway. CX3CL1 plays an important role in immune response, inflammation, cell adhesion and chemotaxis. Fractalkine/CX3CL1 Protein, Human (Biotinylated, HEK293, His, Avi) is the recombinant human-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-Avi, C-10*His labeled tag.

Background

Fractalkine/CX3CL1 is a chemokine that acts as a ligand to CX3CR1 and the integrins ITGAV: ITGB3 and ITGA4: ITGB1. CX3CR1-CX3CL1 signaling performs different functions in different tissue comparators, such as immune response, inflammation, cell adhesion, and chemotaxis. CX3CL1 regulates endothelial cell adhesion and migration and activates integrins through CX3Cr1-dependent and CXCr1-independent activation. CX3CL1 promotes lung cancer cell migration and invasion through the Src/FAK signaling pathway[1][2][3].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human CX3CL1 antibody at 1μg/ml (100μl/well) can bind biotinylated CX3CL1. The ED50 for this effect is 3.504 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-10*His
  • Accession

    P78423/AAH01163 (Q25-Q341)

  • Gene ID
    6376  [NCBI]
  • Protein Length

    Extracellular Domain

  • Conjugation

    Biotin

  • Synonyms

    CX3CL1; NTN; Prev. SCYD1; CX3C Membrane-Anchored Chemokine; Chemokine (C-X3-C Motif) Ligand 1; Small-Inducible Cytokine D1; Fractalkine; C-X3-C Motif Chemokine 1; Neurotactin; NTT; C3Xkine; Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member-1; ABCD

  • AA Sequence

    QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRRQ

  • Predicted Molecular Mass

    38.5 kDa

  • Molecular Weight

    Approximately 50-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
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Volume (start) Volume (start)
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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Fractalkine/CX3CL1 Protein, Human (Biotinylated, HEK293, His, Avi) Product Image