Art. -Nr.: HY-N1476

Heterophyllin B

5 Cited Publications

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Based on 5 publication(s) in Google Scholar

Heterophyllin B is an active cyclic peptide isolated from Pseudostellaria heterophylla. Heterophyllin B provides a novel strategy for the treatment of esophageal cancer.

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Reinheit: 99.93%
CAS. Nr.: 145459-19-4
Formel: C₄₀H₅₈N₈O₈
Molecular Weight:778.94

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Heterophyllin B Chemical Structure

CAS. Nr.: 145459-19-4

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Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (5)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Heterophyllin B is an active cyclic peptide isolated from Pseudostellaria heterophylla. Heterophyllin B provides a novel strategy for the treatment of esophageal cancer^[1].

Alle PI3K Isoform-spezifische Produkte anzeigen :

In Vitro

Heterophyllin B (0-200 µM; 12-48 hours) reduces cell viability in the 75, 100 and 200 µM groups, has no effect on 5, 10, 25 and 50 µM groups^[1].
Heterophyllin B (0-50 µM; 6 hours) suppresses the expression levels of p-PI3K, p-AKT and β-catenin in a dose-dependent manner in ECA-109 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

ECA-109 cells
Concentration:

5 µM, 10 µM, 25 µM, 50 µM, 75 µM, 100 µM and 200 µM
Incubation Time:

12, 24 and 48 hours
Result:

Decreased cell viability at high concentrations.

Western Blot Analysis^[1]

Cell Line:

ECA-109 cells
Concentration:

0 µM, 10 µM, 25 µM, 50 µM
Incubation Time:

6 hours
Result:

Decreased the phosphorylation of PI3K/AKT and the expression of β-catenin.

Chemical Information

CAS. Nr. 145459-19-4
Appearance Solid
Molecular Weight 778.94
Formel C₄₀H₅₈N₈O₈
Color White to off-white
SMILES

O=C([C@@](CCC1)([H])N1C([C@](NC(CNC(CNC2=O)=O)=O)([H])CC(C)C)=O)N(CCC3)[C@]3([H])C(N(CCC4)[C@]4([H])C(N[C@@](C(N[C@H]2CC5=CC=CC=C5)=O)([H])[C@@H](C)CC)=O)=O
Structure Classification
- Cyclopeptides
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (5)

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Journal Impact Factor

Int J Biol Sci

Heterophyllin B enhances transcription factor EB-mediated autophagy and alleviates pyroptosis and oxidative stress after spinal cord injury. [Abstract]

2024 Oct 7;20(14):5415-5435. PMID: 39494322
Sci Rep

HB reduces inflammation in HUVECs via the Nrf2/HO-1 axis by inhabiting apoptosis and oxidative stress. [Abstract]

2026 Jun 23. PMID: 42336938
Tissue Cell

Anti-neoplastic effect of heterophyllin B on ovarian cancer via the regulation of NRF2/HO-1 in vitro and in vivo. [Abstract]

2024 Dec:91:102566. PMID: 39341007
Tissue Cell

Heterophyllin B ameliorates gastric cancer tumor growth through activating ER stress. [Abstract]

2023 Aug:83:102129. PMID: 37406539
Hum Cell

Heterophyllin B inhibits the malignant phenotypes of gastric cancer cells via CXCR4. [Abstract]

2023 Mar;36(2):676-688. PMID: 36539682

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (128.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2838 mL

6.4190 mL

12.8380 mL

5 mM

0.2568 mL

1.2838 mL

2.5676 mL

10 mM

0.1284 mL

0.6419 mL

1.2838 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tantai JC, et al. Heterophyllin B inhibits the adhesion and invasion of ECA-109 human esophageal carcinoma cells by targeting PI3K/AKT/β-catenin signaling. Mol Med Rep. 2016 Feb;13(2):1097-104. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2838 mL	6.4190 mL	12.8380 mL	32.0949 mL
	5 mM	0.2568 mL	1.2838 mL	2.5676 mL	6.4190 mL
	10 mM	0.1284 mL	0.6419 mL	1.2838 mL	3.2095 mL
	15 mM	0.0856 mL	0.4279 mL	0.8559 mL	2.1397 mL
	20 mM	0.0642 mL	0.3209 mL	0.6419 mL	1.6047 mL
	25 mM	0.0514 mL	0.2568 mL	0.5135 mL	1.2838 mL
	30 mM	0.0428 mL	0.2140 mL	0.4279 mL	1.0698 mL
	40 mM	0.0321 mL	0.1605 mL	0.3209 mL	0.8024 mL
	50 mM	0.0257 mL	0.1284 mL	0.2568 mL	0.6419 mL
	60 mM	0.0214 mL	0.1070 mL	0.2140 mL	0.5349 mL
	80 mM	0.0160 mL	0.0802 mL	0.1605 mL	0.4012 mL
	100 mM	0.0128 mL	0.0642 mL	0.1284 mL	0.3209 mL

Keywords

Heterophyllin B

5 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Heterophyllin B

Alle PI3K Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (5)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Heterophyllin B Related Classifications

HY-N1476 Related Classifications