2. Anti-infection Apoptosis
  3. Bacterial Antibiotic Apoptosis Necroptosis
  4. Chlorhexidine (digluconate)

Chlorhexidine digluconate is a chlorophenyl biguanide with broad antibacterial action against both Gram (+) and (-) bacteria and fungi. Chlorhexidine digluconate is a broad-spectrum antiseptic and disinfectant. Chlorhexidine digluconate is effective to prevent and control infectious diseases of the mouth by killing bacteria in saliva and tongue. Chlorhexidine digluconate is a cytotoxic agent and induces cell necrosis and apoptosis.

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Chlorhexidine (digluconate) Chemical Structure

Chlorhexidine (digluconate) Chemical Structure

CAS No. : 18472-51-0

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Solvent
5 g (222.8 mM * 25 mL in Water) USD 50 In-stock
Solvent
20 g (222.8 mM * 100 mL in Water) USD 80 In-stock
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Based on 7 publication(s) in Google Scholar

Other Forms of Chlorhexidine (digluconate):

Chlorhexidine (digluconate) Related Antibodies

Top Publications Citing Use of Products

    Chlorhexidine (digluconate) purchased from MedChemExpress. Usage Cited in: Cancer Res. 2019 Feb 1;79(3):534-545.  [Abstract]

    The half-life of p53 protein is detected in GYS2 overexpressed cells by added with Chlorhexidine (CHX, 20 μg/mL) for different times.

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    Description

    Chlorhexidine digluconate is a chlorophenyl biguanide with broad antibacterial action against both Gram (+) and (-) bacteria and fungi. Chlorhexidine digluconate is a broad-spectrum antiseptic and disinfectant. Chlorhexidine digluconate is effective to prevent and control infectious diseases of the mouth by killing bacteria in saliva and tongue. Chlorhexidine digluconate is a cytotoxic agent and induces cell necrosis and apoptosis[1][2][3].

    In Vitro

    Chlorhexidine digluconate (0.00001%, 0.00005%, 0.0001%, 0.0005%; 1-4 h) demonstrates a cytotoxic effect on CHO cells. Chlorhexidine digluconate reduces the activity of dehydrogenase of CHO cells in a dose-dependent and a time-dependent manner[2].
    Chlorhexidine digluconate (0.00005%, 0.0001%, 0.0005%; 1 h) causes the amount of cell apoptosis of about 1.62%, 5.51%, and 5.47%, respectively. The amount of cell necrosis was 2.38%, 8.62%, and 22.50%, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Chlorhexidine (digluconate) Related Antibodies

    Cell Cytotoxicity Assay[2]

    Cell Line: Chinese hamster ovary cells
    Concentration: 0.00001%, 0.00005%, 0.0001%, 0.0005%
    Incubation Time: 1, 2, 4 h
    Result: Demonstrated a cytotoxic effect on CHO cells.

    Apoptosis Analysis[2]

    Cell Line: Chinese hamster ovary cells
    Concentration: 0.00005%, 0.0001%, 0.0005%
    Incubation Time: 1 h
    Result: After a 1 hour treatment, the amount of cell apoptosis was about 1.62%, 5.51%, and 5.47%, respectively.
    The amount of cell necrosis was 2.38%, 8.62%, and 22.50%, respectively.
    In Vivo

    Chlorhexidine gluconate (0.05%, 0.025%; One-time intraperitoneal injection) with 0.05% lavage has significantly lower incidence of postoperative intra-abdominal abscesses compared with the mice that had saline lavage only[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Imprinting Control Region (ICR) mice at 6 to 8-wk-old (body weight, 25g)[1]
    Dosage: Chlorhexidine gluconate 0.05% and 0.025%
    Administration: One-time intraperitoneal injection
    Result: With 0.05% lavage had significantly lower incidence of postoperative intra-abdominal abscesses compared with the mice that had saline lavage only.
    Clinical Trial
    Molecular Weight

    897.76

    Formula

    C34H54Cl2N10O14
    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    N=C(NC(NCCCCCCNC(NC(NC1=CC=C(Cl)C=C1)=N)=N)=N)NC2=CC=C(Cl)C=C2.O[C@H]([C@@H](O)C(O)=O)[C@H](O)[C@H](O)CO.O[C@H]([C@@H](O)C(O)=O)[C@H](O)[C@H](O)CO
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Solution, -20°C, 2 years
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (111.39 mM; Need ultrasonic)

    DMSO : ≥ 38 mg/mL (42.33 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (111.39 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: ≥98.0%

    COA (251 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.1139 mL 5.5694 mL 11.1388 mL 27.8471 mL
    5 mM 0.2228 mL 1.1139 mL 2.2278 mL 5.5694 mL
    10 mM 0.1114 mL 0.5569 mL 1.1139 mL 2.7847 mL
    15 mM 0.0743 mL 0.3713 mL 0.7426 mL 1.8565 mL
    20 mM 0.0557 mL 0.2785 mL 0.5569 mL 1.3924 mL
    25 mM 0.0446 mL 0.2228 mL 0.4456 mL 1.1139 mL
    30 mM 0.0371 mL 0.1856 mL 0.3713 mL 0.9282 mL
    40 mM 0.0278 mL 0.1392 mL 0.2785 mL 0.6962 mL
    H2O 50 mM 0.0223 mL 0.1114 mL 0.2228 mL 0.5569 mL
    60 mM 0.0186 mL 0.0928 mL 0.1856 mL 0.4641 mL
    80 mM 0.0139 mL 0.0696 mL 0.1392 mL 0.3481 mL
    100 mM 0.0111 mL 0.0557 mL 0.1114 mL 0.2785 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Chlorhexidine (digluconate) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Chlorhexidine (digluconate)18472-51-0BacterialAntibioticApoptosisNecroptosischlorophenyl biguanideGramantisepticdisinfectantmouthsalivatonguecytotoxic agentInhibitorinhibitorinhibit

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