Chlorhexidine diacetate

Name: Chlorhexidine diacetate
Brand: MedChemExpress
SKU: HY-W013699
Rating: 5.0 (7 reviews)

Cat. No.: HY-W013699 Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Chlorhexidine diacetate is a biguanide disinfectant with rapid bactericidal activity against both Gram-positive and Gram-negative organism. The antibacterial effect of chlorhexidine diacetate is related to its action on the bacterial cell membrane and to precipitation of intracellular contents.

For research use only. We do not sell to patients.

Chlorhexidine diacetate Chemical Structure

CAS No. : 56-95-1

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100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Chlorhexidine diacetate:

6 Publications Citing Use of MCE Chlorhexidine diacetate

: Chlorhexidine diacetate purchased from MedChemExpress. Usage Cited in: Cancer Res. 2019 Feb 1;79(3):534-545. [Abstract]; The half-life of p53 protein is detected in GYS2 overexpressed cells by added with Chlorhexidine (CHX, 20 μg/mL) for different times.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Clinical Trial

Molecular Weight

625.55

Formula

C₂₆H₃₈Cl₂N₁₀O₄

CAS No.

56-95-1

Appearance

Solid

Color

White to off-white

SMILES

N=C(NC1=CC=C(Cl)C=C1)NC(NCCCCCCNC(NC(NC2=CC=C(Cl)C=C2)=N)=N)=N.CC(O)=O.CC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

Ethanol : 55 mg/mL (87.92 mM; Need ultrasonic)

H₂O : 20 mg/mL (31.97 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5986 mL	7.9930 mL	15.9859 mL
5 mM	0.3197 mL	1.5986 mL	3.1972 mL
10 mM	0.1599 mL	0.7993 mL	1.5986 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (269 KB) SDS (563 KB)

COA (244 KB) HNMR (294 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Sanchez IR, et al. Chlorhexidine diacetate and povidone-iodine cytotoxicity to canine embryonic fibroblasts and Staphylococcus aureus. Vet Surg. 1988;17(4):182-185. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / Ethanol	1 mM	1.5986 mL	7.9930 mL	15.9859 mL	39.9648 mL
	5 mM	0.3197 mL	1.5986 mL	3.1972 mL	7.9930 mL
	10 mM	0.1599 mL	0.7993 mL	1.5986 mL	3.9965 mL
	15 mM	0.1066 mL	0.5329 mL	1.0657 mL	2.6643 mL
	20 mM	0.0799 mL	0.3996 mL	0.7993 mL	1.9982 mL
	25 mM	0.0639 mL	0.3197 mL	0.6394 mL	1.5986 mL
	30 mM	0.0533 mL	0.2664 mL	0.5329 mL	1.3322 mL
Ethanol	40 mM	0.0400 mL	0.1998 mL	0.3996 mL	0.9991 mL
	50 mM	0.0320 mL	0.1599 mL	0.3197 mL	0.7993 mL
	60 mM	0.0266 mL	0.1332 mL	0.2664 mL	0.6661 mL
	80 mM	0.0200 mL	0.0999 mL	0.1998 mL	0.4996 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.