ASPN Protein, Human (His-SUMO)

ASPN protein acts as a negative regulator in periodontal ligament (PDL) differentiation and mineralization, preventing ossification and maintaining homeostasis of the tooth support system. It inhibits BMP2-induced cell differentiation by blocking the binding of BMP2 to BMPR1B, thereby preventing BMP-dependent activation of SMAD proteins. ASPN Protein, Human (His-SUMO) is the recombinant human-derived ASPN protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of ASPN Protein, Human (His-SUMO) is 348 a.a., with molecular weight of ~60 kDa.

ASPN protein serves as a negative regulator in the differentiation and mineralization processes of the periodontal ligament (PDL), ensuring the prevention of ossification and maintaining the homeostasis of the tooth-supporting system. Its inhibitory action involves blocking BMP2-induced cytodifferentiation of PDL cells by preventing BMP2 binding to the BMPR1B/BMP type-1B receptor, thereby impeding BMP-dependent activation of SMAD proteins. Beyond its role in the PDL, ASPN is a critical regulator of TGF-beta in articular cartilage, where it plays a pivotal role in cartilage homeostasis and contributes to the pathogenesis of osteoarthritis (OA) by negatively regulating chondrogenesis. Furthermore, ASPN interacts with TGFB1, TGFB2, and TGFB3, inhibiting their binding, and engages in calcium binding, participating in osteoblast-driven collagen biomineralization activity. Its intricate interactions with BMP2, type II collagen, and type I collagen underscore its multifaceted involvement in cellular processes that maintain tissue integrity and regulate bone development.

Human

E. coli

N-6*His;N-SUMO

Q9BXN1 (D33-M380)

54829  [NCBI]

DMEDTDDDDDDDDDDDDDDEDNSLFPTREPRSHFFPFDLFPMCPFGCQCYSRVVHCSDLGLTSVPTNIPFDTRMLDLQNNKIKEIKENDFKGLTSLYGLILNNNKLTKIHPKAFLTTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHENKVKKIQKDTFKGMNALHVLEMSANPLDNNGIEPGAFEGVTVFHIRIAEAKLTSVPKGLPPTLLELHLDYNKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNKLKKIPSGLPELKYLQIIFLHSNSIARVGVNDFCPTVPKMKKSLYSAISLFNNPVKYWEMQPATFRCVLSRMSVQLGNFGM

Approximately 60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.