eGFP mRNA-LNP (500 ng/μL)
Based on 1 Customer Validation
eGFP mRNA-LNP (500 ng/μL) is a lipid nanoparticle (LNP) containing eGFP mRNA. eGFP mRNA-LNP carries Enhanced Green Fluorescent Protein (eGFP), which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry (excitation wavelength = 488 nm; emission wavelength = 535/40 bandpass). eGFP mRNA-LNP can be used for RNA delivery, translation efficiency and cell viability.
For research use only. We do not sell to patients.
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Storage:
-80°C
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
eGFP mRNA-LNP (500 ng/μL) (1 μM, 24 h) results in full mRNA formulation translation into eGFP protein at significant amounts in HeLa cell line, whereas the translation by macrophages is low[2].
eGFP mRNA-LNP (1 μM, 24 h) results in weak signals in Kupffer cells (KC) , whereas hepatocytes expresses eGFP in visible amounts throughout the whole cell[2].
eGFP mRNA-LNP (0.25-1 μM, 24-48 h) shows time-dependent eGFP transfection at 0.25 μM, and time does not make much of a difference at 1 μM in alveolar epithelial cells and human primary lung fibroblasts[3].
eGFP mRNA-LNP (0.4 μM, 48 h) shows over half of the cell lines exhibit medium or poor transfection whilst human colon epithelial HCT116 and human hepatocyte HuH-7 cells are the high expressors[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hepatocytes
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Concentration:1 μM
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Incubation Time:24 h
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Result:Had a minor impact on the cell cycle progression of hepatocyte after transfection.
Did not affect the pattern of hepatocyte subsets.
Showed that big hepatocytes expressed the highest levels of eGFP.
eGFP mRNA-LNP (500 ng/μL) (3 mg/kg, i.v., single dose) allows significant and detectable eGFP protein expression level in Kupffer cells (KC) and monocytic macrophages (MoMϕ) in C57Bl/6J[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL6J wild type mice[2]
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Dosage:2 mg/kg
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Administration:Intravenous injection (i.v.), single dose
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Result:Resulted in varied protein translation intensity between different hepatocytes, larger hepatocytes exhibited stronger signals than smaller ones.
Did not detect obvious signal in any other hepatic cell type.
Resulted in smaller and rounder KC compared to control mice, where the KC appeared to be elongated.
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Animal Model:C57BL6J wild type mice[2]
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Dosage:3 mg/kg
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Administration:Intravenous injection (i.v.), single dose
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Result:Displayed a pronounced autofluorescence in HSC and hepatocytes of mice.
Showed significant amounts were taken up by hepatocytes and KC but not HSC.
Chemical Information
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[eGFP mRNA-LNP (500 ng/μL)]
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Shipping
Shipping with dry ice.
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Storage
-80°C
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Skelton D, et al. The enhanced green fluorescent protein (eGFP) is minimally immunogenic in C57BL/6 mice. Gene Ther. 2001 Dec;8(23):1813-4. [Content Brief]
[2]. Lin, C., et al., (2025). Exceptional Uptake, Limited Protein Expression: Liver Macrophages Lost in Translation of Synthetic mRNA. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12(9), e2409729. [Content Brief]
[3]. Massaro, M., et al., (2023). Lipid nanoparticle-mediated mRNA delivery in lung fibrosis. European journal of pharmaceutical sciences: official journal of the European Federation for Pharmaceutical Sciences, 183, 106370. [Content Brief]
[4]. Sayers, E. J., et al., (2019). Endocytic Profiling of Cancer Cell Models Reveals Critical Factors Influencing LNP-Mediated mRNA Delivery and Protein Expression. Molecular therapy : the journal of the American Society of Gene Therapy, 27(11), 1950-1962. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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