Enpp/Carbonic anhydrase-IN-2
Based on 1 Customer Validation
Enpp/Carbonic anhydrase-IN-2 (compound 1i) is a potent Enpp and carbonic anhydrase inhibitor with IC50s of 1.13, 1.07, 0.74, 0.33, 0.68 for NPP1, NPP2, NPP3, CA-IX, CA-XII respectively. Enpp/Carbonic anhydrase-IN-2 shows antiproliferative activity for cancer cells and low cytotoxic against normal cells. Enpp/Carbonic anhydrase-IN-2 induces Apoptosis.
For research use only. We do not sell to patients.
- Purity: 98.48%
- CAS No.: 2883495-39-2
- Formula: C23H24FNO4S
- Molecular Weight:429.50
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
|
CA IX 0.33 μM (IC50) |
CA XII 0.68 μM (IC50) |
NPP1 1.13 μM (IC50) |
NPP2 1.07 μM (IC50) |
NPP3 0.74 μM (IC50) |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 786-0 | IC50 |
5.08 μM
Compound: 1i
|
Cytotoxicity against human 786-0 cells assessed as cell growth inhibition
Cytotoxicity against human 786-0 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| A498 | IC50 |
0.28 μM
Compound: 1i
|
Cytotoxicity against human A498 cells assessed as cell growth inhibition
Cytotoxicity against human A498 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| A549 | IC50 |
2.3 μM
Compound: 1i
|
Cytotoxicity against human A549 cells assessed as cell growth inhibition
Cytotoxicity against human A549 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| ACHN | IC50 |
15.7 μM
Compound: 1i
|
Cytotoxicity against human ACHN cells assessed as cell growth inhibition
Cytotoxicity against human ACHN cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| BT-549 | IC50 |
4.28 μM
Compound: 1i
|
Cytotoxicity against human BT-549 cells assessed as cell growth inhibition
Cytotoxicity against human BT-549 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| CCRF-CEM | IC50 |
3.73 μM
Compound: 1i
|
Cytotoxicity against human CCRF-CEM cells assessed as cell growth inhibition
Cytotoxicity against human CCRF-CEM cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| COLO 205 | IC50 |
0.58 μM
Compound: 1i
|
Cytotoxicity against human COLO 205 cells assessed as cell growth inhibition
Cytotoxicity against human COLO 205 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| DU-145 | IC50 |
10.6 μM
Compound: 1i
|
Cytotoxicity against human DU-145 cells assessed as cell growth inhibition
Cytotoxicity against human DU-145 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| HCC 2998 | IC50 |
7.95 μM
Compound: 1i
|
Cytotoxicity against human HCC 2998 cells assessed as cell growth inhibition
Cytotoxicity against human HCC 2998 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| HCT-116 | IC50 |
0.63 μM
Compound: 1i
|
Cytotoxicity against human HCT-116 cells assessed as cell growth inhibition
Cytotoxicity against human HCT-116 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| HCT-15 | IC50 |
0.42 μM
Compound: 1i
|
Cytotoxicity against human HCT-15 cells assessed as cell growth inhibition
Cytotoxicity against human HCT-15 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| HL-60(TB) | IC50 |
2.68 μM
Compound: 1i
|
Cytotoxicity against human HL-60(TB) cells assessed as cell growth inhibition
Cytotoxicity against human HL-60(TB) cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| HOP-62 | IC50 |
11.1 μM
Compound: 1i
|
Cytotoxicity against human HOP-62 cells assessed as cell growth inhibition
Cytotoxicity against human HOP-62 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| HOP-92 | IC50 |
1.06 μM
Compound: 1i
|
Cytotoxicity against human HOP-92 cells assessed as cell growth inhibition
Cytotoxicity against human HOP-92 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| Hs-578T | IC50 |
2.29 μM
Compound: 1i
|
Cytotoxicity against human Hs-578T cells assessed as cell growth inhibition
Cytotoxicity against human Hs-578T cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| HT-29 | IC50 |
0.2 μM
Compound: 1i
|
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| IGROV-1 | IC50 |
10.4 μM
Compound: 1i
|
Cytotoxicity against human IGROV-1 cells assessed as cell growth inhibition
Cytotoxicity against human IGROV-1 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| K562 | IC50 |
0.58 μM
Compound: 1i
|
Cytotoxicity against human K562 cells assessed as cell growth inhibition
Cytotoxicity against human K562 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| KM12 | IC50 |
0.72 μM
Compound: 1i
|
Cytotoxicity against human KM12 cells assessed as cell growth inhibition
Cytotoxicity against human KM12 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| LOX IMVI | IC50 |
7.57 μM
Compound: 1i
|
Cytotoxicity against human LOX IMVI cells assessed as cell growth inhibition
Cytotoxicity against human LOX IMVI cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| M14 | IC50 |
6.02 μM
Compound: 1i
|
Cytotoxicity against human M14 cells assessed as cell growth inhibition
Cytotoxicity against human M14 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| Malme-3M | IC50 |
4.07 μM
Compound: 1i
|
Cytotoxicity against human Malme-3M cells assessed as cell growth inhibition
Cytotoxicity against human Malme-3M cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| MCF7 | IC50 |
0.59 μM
Compound: 1i
|
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| MDA-MB-231 | IC50 |
1.87 μM
Compound: 1i
|
Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition
Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| MDA-MB-435 | IC50 |
7.8 μM
Compound: 1i
|
Cytotoxicity against human MDA-MB-435 cells assessed as cell growth inhibition
Cytotoxicity against human MDA-MB-435 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| MDA-MB-468 | IC50 |
1.01 μM
Compound: 1i
|
Cytotoxicity against human MDA-MB-468 cells assessed as cell growth inhibition
Cytotoxicity against human MDA-MB-468 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| MOLT-4 | IC50 |
5.05 μM
Compound: 1i
|
Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition
Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| NCI/ADR-RES | IC50 |
0.94 μM
Compound: 1i
|
Cytotoxicity against human NCI/ADR-RES cells assessed as cell growth inhibition
Cytotoxicity against human NCI/ADR-RES cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| NCI-H226 | IC50 |
19.2 μM
Compound: 1i
|
Cytotoxicity against human NCI-H226 cells assessed as cell growth inhibition
Cytotoxicity against human NCI-H226 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| NCI-H23 | IC50 |
7.71 μM
Compound: 1i
|
Cytotoxicity against human NCI-H23 cells assessed as cell growth inhibition
Cytotoxicity against human NCI-H23 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| NCI-H322M | IC50 |
5.55 μM
Compound: 1i
|
Cytotoxicity against human NCI-H322M cells assessed as cell growth inhibition
Cytotoxicity against human NCI-H322M cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| NCI-H460 | IC50 |
2.3 μM
Compound: 1i
|
Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition
Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| NCI-H522 | IC50 |
3.39 μM
Compound: 1i
|
Cytotoxicity against human NCI-H522 cells assessed as cell growth inhibition
Cytotoxicity against human NCI-H522 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| OVCAR-3 | IC50 |
6.05 μM
Compound: 1i
|
Cytotoxicity against human OVCAR-3 cells assessed as cell growth inhibition
Cytotoxicity against human OVCAR-3 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| OVCAR-4 | IC50 |
1.61 μM
Compound: 1i
|
Cytotoxicity against human OVCAR-4 cells assessed as cell growth inhibition
Cytotoxicity against human OVCAR-4 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| OVCAR-5 | IC50 |
28.8 μM
Compound: 1i
|
Cytotoxicity against human OVCAR-5 cells assessed as cell growth inhibition
Cytotoxicity against human OVCAR-5 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| OVCAR-8 | IC50 |
12.8 μM
Compound: 1i
|
Cytotoxicity against human OVCAR-8 cells assessed as cell growth inhibition
Cytotoxicity against human OVCAR-8 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| PC-3 | IC50 |
0.49 μM
Compound: 1i
|
Cytotoxicity against human PC-3 cells assessed as cell growth inhibition
Cytotoxicity against human PC-3 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| RPMI-8226 | IC50 |
2.15 μM
Compound: 1i
|
Cytotoxicity against human RPMI-8226 cells assessed as cell growth inhibition
Cytotoxicity against human RPMI-8226 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| RXF 393 | IC50 |
4.76 μM
Compound: 1i
|
Cytotoxicity against human RXF 393 cells assessed as cell growth inhibition
Cytotoxicity against human RXF 393 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SF-268 | IC50 |
20.8 μM
Compound: 1i
|
Cytotoxicity against human SF-268 cells assessed as cell growth inhibition
Cytotoxicity against human SF-268 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SF-295 | IC50 |
1.81 μM
Compound: 1i
|
Cytotoxicity against human SF-295 cells assessed as cell growth inhibition
Cytotoxicity against human SF-295 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SF-539 | IC50 |
0.76 μM
Compound: 1i
|
Cytotoxicity against human SF-539 cells assessed as cell growth inhibition
Cytotoxicity against human SF-539 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SK-MEL-2 | IC50 |
10.5 μM
Compound: 1i
|
Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition
Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SK-MEL-28 | IC50 |
28.7 μM
Compound: 1i
|
Cytotoxicity against human SK-MEL-28 cells assessed as cell growth inhibition
Cytotoxicity against human SK-MEL-28 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SK-MEL-5 | IC50 |
3.61 μM
Compound: 1i
|
Cytotoxicity against human SK-MEL-5 cells assessed as cell growth inhibition
Cytotoxicity against human SK-MEL-5 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SK-OV-3 | IC50 |
7.83 μM
Compound: 1i
|
Cytotoxicity against human SK-OV-3 cells assessed as cell growth inhibition
Cytotoxicity against human SK-OV-3 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SN12C | IC50 |
19 μM
Compound: 1i
|
Cytotoxicity against human SN12C cells assessed as cell growth inhibition
Cytotoxicity against human SN12C cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SNB-19 | IC50 |
15.7 μM
Compound: 1i
|
Cytotoxicity against human SNB-19 cells assessed as cell growth inhibition
Cytotoxicity against human SNB-19 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SNB-75 | IC50 |
1.97 μM
Compound: 1i
|
Cytotoxicity against human SNB-75 cells assessed as cell growth inhibition
Cytotoxicity against human SNB-75 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SR | IC50 |
3.6 μM
Compound: 1i
|
Cytotoxicity against human SR cells assessed as cell growth inhibition
Cytotoxicity against human SR cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| SW-620 | IC50 |
0.41 μM
Compound: 1i
|
Cytotoxicity against human SW620 cells assessed as cell growth inhibition
Cytotoxicity against human SW620 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| T47D | IC50 |
0.83 μM
Compound: 1i
|
Cytotoxicity against human T47D cells assessed as cell growth inhibition
Cytotoxicity against human T47D cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| TK-10 | IC50 |
20.2 μM
Compound: 1i
|
Cytotoxicity against human TK-10 cells assessed as cell growth inhibition
Cytotoxicity against human TK-10 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| U-251 | IC50 |
19.4 μM
Compound: 1i
|
Cytotoxicity against human U-251 cells assessed as cell growth inhibition
Cytotoxicity against human U-251 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| UACC-257 | IC50 |
3.4 μM
Compound: 1i
|
Cytotoxicity against human UACC-257 cells assessed as cell growth inhibition
Cytotoxicity against human UACC-257 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| UACC-62 | IC50 |
3.14 μM
Compound: 1i
|
Cytotoxicity against human UACC-62 cells assessed as cell growth inhibition
Cytotoxicity against human UACC-62 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
| UO-31 | IC50 |
3.43 μM
Compound: 1i
|
Cytotoxicity against human UO-31 cells assessed as cell growth inhibition
Cytotoxicity against human UO-31 cells assessed as cell growth inhibition
|
[PMID: 36470105] |
Enpp/Carbonic anhydrase-IN-2 (compound 1i) (0-100 µM; ) inhibits some cancer cells growth with IC50s of 0.58, 0.58, 0.63, 0.42, 0.20, 0.72, 0.41, 0.76, 0.94, 0.28, 0.49, 0.59, 0.83 µM for K-562, COLO 205, HCT-116, HCT-15, HT29, KM12, SW-620, SF-539, NCI/ADR-RES, A498, PC-3, MCF7, T-47D cells, respectively[1].
Enpp/Carbonic anhydrase-IN-2 (0-2 µM) shows low cytotoxic against normal breast epithelial cells (HME1) and normal skin fibroblast cells (F180) with IC50s of > 50 μM[1].
Enpp/Carbonic anhydrase-IN-2 (0.58, 1.16 µM) induces apoptosis in a dose-dependent manner at K-562 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:K-562, COLO 205, HCT-116, HCT-15, HT29, KM12, SW-620, SF-539, NCI/ADR-RES, A498, PC-3, MCF7, T-47D cells
-
Concentration:0-100 µM
-
Incubation Time:
-
Result:Inhibited the cell growth with IC50s of 0.58, 0.58, 0.63, 0.42, 0.20, 0.72, 0.41, 0.76, 0.94, 0.28, 0.49, 0.59, 0.83 µM for K-562, COLO 205, HCT-116, HCT-15, HT29, KM12, SW-620, SF-539, NCI/ADR-RES, A498, PC-3, MCF7, T-47D cells, respectively.
-
Cell Line:K-562 cells
-
Concentration:0.58, 1.16 µM
-
Incubation Time:
-
Result:Induced apoptosis in a dose-dependent manner.
Chemical Information
-
CAS No. 2883495-39-2
-
Appearance Solid
-
Molecular Weight 429.50
-
Formula C23H24FNO4S
-
Color White to off-white
-
SMILES
O=C(C12CC3CC(C2)CC(C1)C3)NC4=CC=C(C=C4)OS(=O)(C5=CC=C(C=C5)F)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (232.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3283 mL | 11.6414 mL | 23.2829 mL | 58.2072 mL |
| 5 mM | 0.4657 mL | 2.3283 mL | 4.6566 mL | 11.6414 mL | |
| 10 mM | 0.2328 mL | 1.1641 mL | 2.3283 mL | 5.8207 mL | |
| 15 mM | 0.1552 mL | 0.7761 mL | 1.5522 mL | 3.8805 mL | |
| 20 mM | 0.1164 mL | 0.5821 mL | 1.1641 mL | 2.9104 mL | |
| 25 mM | 0.0931 mL | 0.4657 mL | 0.9313 mL | 2.3283 mL | |
| 30 mM | 0.0776 mL | 0.3880 mL | 0.7761 mL | 1.9402 mL | |
| 40 mM | 0.0582 mL | 0.2910 mL | 0.5821 mL | 1.4552 mL | |
| 50 mM | 0.0466 mL | 0.2328 mL | 0.4657 mL | 1.1641 mL | |
| 60 mM | 0.0388 mL | 0.1940 mL | 0.3880 mL | 0.9701 mL | |
| 80 mM | 0.0291 mL | 0.1455 mL | 0.2910 mL | 0.7276 mL | |
| 100 mM | 0.0233 mL | 0.1164 mL | 0.2328 mL | 0.5821 mL |