Enpp/Carbonic anhydrase-IN-2

Name: Enpp/Carbonic anhydrase-IN-2
Brand: MedChemExpress
SKU: HY-151917
Rating: 4.5 (1 reviews)

Cat. No.: HY-151917 Purity: 99.76%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Enpp/Carbonic anhydrase-IN-2 (compound 1i) is a potent Enpp and carbonic anhydrase inhibitor with IC₅₀s of 1.13, 1.07, 0.74, 0.33, 0.68 for NPP1, NPP2, NPP3, CA-IX, CA-XII respectively. Enpp/Carbonic anhydrase-IN-2 shows antiproliferative activity for cancer cells and low cytotoxic against normal cells. Enpp/Carbonic anhydrase-IN-2 induces Apoptosis.

For research use only. We do not sell to patients.

Enpp/Carbonic anhydrase-IN-2 Chemical Structure

CAS No. : 2883495-39-2

Size	Price	Stock	Quantity
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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•Related Screening Libraries:

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View All Carbonic Anhydrase Isoform Specific Products:

View All Isoforms

CA CA I CA Ⅱ CA Ⅸ CA XII

View All Phosphodiesterase (PDE) Isoform Specific Products:

View All Isoforms

PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

CA Ⅸ

0.33 μM (IC₅₀)

CA XII

0.68 μM (IC₅₀)

NPP1

1.13 μM (IC₅₀)

NPP2

1.07 μM (IC₅₀)

NPP3

0.74 μM (IC₅₀)

In Vitro

Enpp/Carbonic anhydrase-IN-2 (compound 1i) (0-100 µM; ) inhibits some cancer cells growth with IC₅₀s of 0.58, 0.58, 0.63, 0.42, 0.20, 0.72, 0.41, 0.76, 0.94, 0.28, 0.49, 0.59, 0.83 µM for K-562, COLO 205, HCT-116, HCT-15, HT29, KM12, SW-620, SF-539, NCI/ADR-RES, A498, PC-3, MCF7, T-47D cells, respectively^[1].
Enpp/Carbonic anhydrase-IN-2 (0-2 µM) shows low cytotoxic against normal breast epithelial cells (HME1) and normal skin fibroblast cells (F180) with IC₅₀s of > 50 μM^[1].
Enpp/Carbonic anhydrase-IN-2 (0.58, 1.16 µM) induces apoptosis in a dose-dependent manner at K-562 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	K-562, COLO 205, HCT-116, HCT-15, HT29, KM12, SW-620, SF-539, NCI/ADR-RES, A498, PC-3, MCF7, T-47D cells
Concentration:	0-100 µM
Incubation Time:
Result:	Inhibited the cell growth with IC₅₀s of 0.58, 0.58, 0.63, 0.42, 0.20, 0.72, 0.41, 0.76, 0.94, 0.28, 0.49, 0.59, 0.83 µM for K-562, COLO 205, HCT-116, HCT-15, HT29, KM12, SW-620, SF-539, NCI/ADR-RES, A498, PC-3, MCF7, T-47D cells, respectively.

Apoptosis Analysis^[1]

Cell Line:	K-562 cells
Concentration:	0.58, 1.16 µM
Incubation Time:
Result:	Induced apoptosis in a dose-dependent manner.

Molecular Weight

429.50

Formula

C₂₃H₂₄FNO₄S

CAS No.

2883495-39-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(C12CC3CC(C2)CC(C1)C3)NC4=CC=C(C=C4)OS(=O)(C5=CC=C(C=C5)F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (232.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3283 mL	11.6414 mL	23.2829 mL
5 mM	0.4657 mL	2.3283 mL	4.6566 mL
10 mM	0.2328 mL	1.1641 mL	2.3283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (252 KB) HNMR (248 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Afnan I.Shahin, et al. Design and synthesis of new adamantyl derivatives as promising antiproliferative agents. European Journal of Medicinal Chemistry, 2022.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3283 mL	11.6414 mL	23.2829 mL	58.2072 mL
	5 mM	0.4657 mL	2.3283 mL	4.6566 mL	11.6414 mL
	10 mM	0.2328 mL	1.1641 mL	2.3283 mL	5.8207 mL
	15 mM	0.1552 mL	0.7761 mL	1.5522 mL	3.8805 mL
	20 mM	0.1164 mL	0.5821 mL	1.1641 mL	2.9104 mL
	25 mM	0.0931 mL	0.4657 mL	0.9313 mL	2.3283 mL
	30 mM	0.0776 mL	0.3880 mL	0.7761 mL	1.9402 mL
	40 mM	0.0582 mL	0.2910 mL	0.5821 mL	1.4552 mL
	50 mM	0.0466 mL	0.2328 mL	0.4657 mL	1.1641 mL
	60 mM	0.0388 mL	0.1940 mL	0.3880 mL	0.9701 mL
	80 mM	0.0291 mL	0.1455 mL	0.2910 mL	0.7276 mL
	100 mM	0.0233 mL	0.1164 mL	0.2328 mL	0.5821 mL