Levamisole
Based on 17 publication(s) in Google Scholar
Levamisole ((-)-Levamisole), an anthelmintic agent with immunomodulatory properties. Levamisole acts as a positive allosteric modulator (PAM) for the α3β2 (EC50=300 μM) and α3β4 (EC50=100 μM) subtype of nAChRs. Orally active.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.96%
- No. CAS: 14769-73-4
- Fòrmula: C11H12N2S
- Peso molecular:204.30
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Levamisole
More- EBioMedicine. 2025 Sep:119:105914. [Abstract]
- ACS Environ Au. 2025 Aug 5;5(6):573-582. [Abstract]
- Int J Biol Macromol. 2025 Apr 17;310(Pt 1):143312. [Abstract]
- Emerg Contam. 2026 Feb 23.
- Food Sci Hum Wellness. 2026 Apr 7.
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- J Ethnopharmacol. 2024 Oct 28:333:118406. [Abstract]
- Geroscience. 2026 Mar 5. [Abstract]
- Biomolecules. 2026 Mar 25;16(4):496. [Abstract]
- FEBS J. 2026 Apr 8. [Abstract]
- J Chromatogr B Analyt Technol Biomed Life Sci. 2026 May 16:1280:125125. [Abstract]
- Rapid Commun Mass Spectrom. 2023 Feb 15;37(3):e9430. [Abstract]
- bioRxiv. 2025 Nov 1.
- Phytomed Plus. 2025 Aug 23.
- bioRxiv. 2025 Jul 24.
- bioRxiv. 2023 Oct 15.
- Environ Sci Pollut Res Int. 2023 Jan;30(5):12522-12531. [Abstract]
Customer Validation & Images
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Histological Imaging/Staining
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>100 μM
Compound: Levamisole
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Cytotoxicity against human A549 cells assessed as cell growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as cell growth inhibition after 24 hrs by MTT assay
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[PMID: 27376493] |
| CCRF-CEM | IC50 |
>250 μM
Compound: Levamisole
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Cytotoxicity against human CEM cells after 72 hrs by Coulter counting method
Cytotoxicity against human CEM cells after 72 hrs by Coulter counting method
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[PMID: 25064346] |
| HeLa | IC50 |
250 μM
Compound: Levamisole
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Cytotoxicity against human HeLa cells after 72 hrs by Coulter counting method
Cytotoxicity against human HeLa cells after 72 hrs by Coulter counting method
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[PMID: 25064346] |
| HeLa | IC50 |
96 μM
Compound: Levamisole
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Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 24 hrs by MTT assay
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[PMID: 27376493] |
| L1210 | IC50 |
206 μM
Compound: Levamisole
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Cytotoxicity against mouse L1210 cells after 48 hrs by Coulter counting method
Cytotoxicity against mouse L1210 cells after 48 hrs by Coulter counting method
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[PMID: 25064346] |
| MCF7 | IC50 |
>100 μM
Compound: Levamisole
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Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 24 hrs by MTT assay
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[PMID: 27376493] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 14769-73-4
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Appearance Solid
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Peso molecular 204.30
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Fòrmula C11H12N2S
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Color White to off-white
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SMILES
C12=N[C@@H](C3=CC=CC=C3)CN1CCS2
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Synonyms
(-)-Tetramisole
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (17)
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Journal Impact Factor
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Most Recent
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EBioMedicine
A strategy to re-sensitise drug-resistant Gram-positive bacteria to oxazolidinone-class antibiotics. [Abstract]2025 Sep:119:105914. PMID: 40912150 -
ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Int J Biol Macromol
An inulin-type fructan from Codonopsis pilosula ameliorates cyclophosphamide-induced immunosuppression and intestinal barrier injury in mice. [Abstract]2025 Apr 17;310(Pt 1):143312. PMID: 40250123
Levamisole purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Apr 17;310(Pt 1):143312. [Abstract]
Levamisole hydrochloride (LMS: 40 mg/kg). Serum levels of IL-2, TNF-α, and IFN-γ.
Levamisole purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Apr 17;310(Pt 1):143312. [Abstract]
Levamisole hydrochloride (LMS: 40 mg/kg). Intestinal H&E staining.
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
J Ethnopharmacol
2024 Oct 28:333:118406. PMID: 38838923 -
Geroscience
2026 Mar 5. PMID: 41781498 -
Biomolecules
Immunomodulatory Effects of Periplaneta americana Oligosaccharides Through SCFA-Producing Gut Microbiota and Metabolic Regulation in Immunosuppressed Mice. [Abstract]2026 Mar 25;16(4):496. PMID: 42072618 -
FEBS J
Compromised DNA replication in gut cells underlies sensitivity to genotoxic stress in the tardigrade Hypsibius exemplaris. [Abstract]2026 Apr 8. PMID: 41949121 -
J Chromatogr B Analyt Technol Biomed Life Sci
A quantifiable grading standard for Codonopsis Radix based on the multidimensional quality evaluation system of phenotype, chemistry, and bioactivity. [Abstract]2026 May 16:1280:125125. PMID: 42143989 -
Rapid Commun Mass Spectrom
Hydroxy levamisole and its phase II conjugates as potential indicators of levamisole doping in thoroughbred horses. [Abstract]2023 Feb 15;37(3):e9430. PMID: 36382989 -
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Environ Sci Pollut Res Int
Fosthiazate exposure induces oxidative stress, nerve damage, and reproductive disorders in nontarget nematodes. [Abstract]2023 Jan;30(5):12522-12531. PMID: 36112285
Solvente y solubilidad
DMSO : 100 mg/mL (489.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Lewis JA, et al. Levamisole: A Positive Allosteric Modulator for the α3β4 Nicotinic Acetylcholine Receptors Prevents Weight Gain in the CD-1 Mice on a High Fat Diet. Curr Pharm Des. 2017;23(12):1869-1872. [Content Brief]
[2]. Mehta KP, et al. Immunoregulatory treatment for minimal change nephrotic syndrome. Arch Dis Child. 1986;61(2):153-158. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.8948 mL | 24.4741 mL | 48.9481 mL | 122.3703 mL |
| 5 mM | 0.9790 mL | 4.8948 mL | 9.7896 mL | 24.4741 mL | |
| 10 mM | 0.4895 mL | 2.4474 mL | 4.8948 mL | 12.2370 mL | |
| 15 mM | 0.3263 mL | 1.6316 mL | 3.2632 mL | 8.1580 mL | |
| 20 mM | 0.2447 mL | 1.2237 mL | 2.4474 mL | 6.1185 mL | |
| 25 mM | 0.1958 mL | 0.9790 mL | 1.9579 mL | 4.8948 mL | |
| 30 mM | 0.1632 mL | 0.8158 mL | 1.6316 mL | 4.0790 mL | |
| 40 mM | 0.1224 mL | 0.6119 mL | 1.2237 mL | 3.0593 mL | |
| 50 mM | 0.0979 mL | 0.4895 mL | 0.9790 mL | 2.4474 mL | |
| 60 mM | 0.0816 mL | 0.4079 mL | 0.8158 mL | 2.0395 mL | |
| 80 mM | 0.0612 mL | 0.3059 mL | 0.6119 mL | 1.5296 mL | |
| 100 mM | 0.0489 mL | 0.2447 mL | 0.4895 mL | 1.2237 mL |
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