GDP-azido-fucose disodium
Based on 1 Customer Validation
GDP-azido-fucose disodium is a glycoengineering tool. GDP-azido-fucose disodium modifies complex glycans by adding an azido-fucose group. GDP-azido-fucose disodium can install azido-fucose tags on antibody Fc glycans for site-specific payload conjugation, thereby forming antibody-drug conjugates with defined ratios.
For research use only. We do not sell to patients.
- Purity: 99.53%
- CAS No.: 944560-34-3
- Formula: C16H22N8Na2O15P2
- Molecular Weight:674.32
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
GDP-azido-fucose disodium serves as a substrate for human α-1,3 fucosyltransferase, introducing an azido-fucose tag into the complex glycan G2 to generate glycan G3[1].
GDP-azido-fucose disodium serves as a precursor for fluorophore-conjugated GDP-fucose via CuI-catalyzed azide-alkyne cycloaddition reaction[2].
GDP-azido-fucose disodium generates fluorophore-conjugated GDP-fucose derivatives that serve as functional donor substrates for FUT2, FUT6, FUT7, FUT8 and FUT9. Each enzyme exhibits distinct fluorophore preferences and enables specific labeling of substrate glycans on fetal bovine fetuin, asialofetuin, Cantuzumab (HY-P99967) and NIST monoclonal antibodies[2].
GDP-azido-fucose disodium can generate Alexa Fluor® 555-conjugated GDP-fucose, which enables FUT9- and FUT7-mediated glycoprotein labeling in HEK293 cell extracts. This reveals mutually exclusive substrate recognition between FUT9 and ST6Gal1, with minimal interference between FUT9 and ST3Gal2[2].
GDP-azido-fucose disodium can generate Cy5-conjugated GDP-fucose, which, mediated by FUT8, detects Man5 high-mannose-type glycans on RNase B and Man3 glycans on recombinant 1918 H1N1 influenza neuraminidase, provided that these glycans are first modified by MGAT1 to introduce β-2-linked GlcNAc on the α-3 arm[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 944560-34-3
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Appearance Solid
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Molecular Weight 674.32
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Formula C16H22N8Na2O15P2
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Color White to off-white
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SMILES
O=C1C2=C(N([C@H]3[C@H](O)[C@H](O)[C@@H](COP(OP(O[C@H]4O[C@H]([C@H]([C@H]([C@@H]4O)O)O)CN=[N+]=[N-])(O[Na])=O)(O[Na])=O)O3)C=N2)NC(N)=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
H2O : 50 mg/mL (74.15 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.4830 mL | 7.4149 mL | 14.8298 mL | 37.0744 mL |
| 5 mM | 0.2966 mL | 1.4830 mL | 2.9660 mL | 7.4149 mL | |
| 10 mM | 0.1483 mL | 0.7415 mL | 1.4830 mL | 3.7074 mL | |
| 15 mM | 0.0989 mL | 0.4943 mL | 0.9887 mL | 2.4716 mL | |
| 20 mM | 0.0741 mL | 0.3707 mL | 0.7415 mL | 1.8537 mL | |
| 25 mM | 0.0593 mL | 0.2966 mL | 0.5932 mL | 1.4830 mL | |
| 30 mM | 0.0494 mL | 0.2472 mL | 0.4943 mL | 1.2358 mL | |
| 40 mM | 0.0371 mL | 0.1854 mL | 0.3707 mL | 0.9269 mL | |
| 50 mM | 0.0297 mL | 0.1483 mL | 0.2966 mL | 0.7415 mL | |
| 60 mM | 0.0247 mL | 0.1236 mL | 0.2472 mL | 0.6179 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
GDP-azido-fucose disodium Related Classifications
HY-164169A Related Classifications
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Keywords
- GDP-azido-fucose disodium
- 944560-34-3
- Biochemical Assay Reagents
- Glycosyltransferase
- breast cancer
- human α-1,3 fucosyltransferase
- antibody-drug conjugates
- FuT6
- copper(I)-catalyzed azide-alkyne cycloaddition
- complex-type glycans
- glioblastoma multiforme
- antibody Fc-glycans
- FUT2
- ovarian cancer
- Inhibitor
- inhibitor
- inhibit