GDP-azido-fucose

GDP-azido-fucose serves as a substrate for human α-1,3 fucosyltransferase, introducing an azido-fucose tag into the complex glycan G2 to generate glycan G3^[1].
GDP-azido-fucose serves as a precursor for fluorophore-conjugated GDP-fucose via Cu^I-catalyzed azide-alkyne cycloaddition reaction^[2].
GDP-azido-fucose generates fluorophore-conjugated GDP-fucose derivatives that serve as functional donor substrates for FUT2, FUT6, FUT7, FUT8 and FUT9. Each enzyme exhibits distinct fluorophore preferences and enables specific labeling of substrate glycans on fetal bovine fetuin, asialofetuin, Cantuzumab (HY-P99967) and NIST monoclonal antibodies^[2].
GDP-azido-fucose can generate Alexa Fluor® 555-conjugated GDP-fucose, which enables FUT9- and FUT7-mediated glycoprotein labeling in HEK293 cell extracts. This reveals mutually exclusive substrate recognition between FUT9 and ST6Gal1, with minimal interference between FUT9 and ST3Gal2^[2].
GDP-azido-fucose can generate Cy5-conjugated GDP-fucose, which, mediated by FUT8, detects Man5 high-mannose-type glycans on RNase B and Man3 glycans on recombinant 1918 H1N1 influenza neuraminidase, provided that these glycans are first modified by MGAT1 to introduce β-2-linked GlcNAc on the α-3 arm^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.