H-D-Val-Leu-Lys-AFC TFA
Based on 1 Customer Validation
H-D-Val-Leu-Lys-AFC TFA (Plasmin substrate TFA) is a plasmin-specific fluorescent substrate. H-D-Val-Leu-Lys-AFC TFA can be cleaved to generate a fluorescent signal, which is used to determine plasmin activity. Abs/Em=380/500 nm.
For research use only. We do not sell to patients.
- Purity : 98.79%
- Formula: C27H38F3N5O5·xC2HF3O2
- Molecular Weight:569.62 (free base)
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Biological Activity
Description
In Vitro
H-D-Val-Leu-Lys-AFC TFA (6 μM; 30 min) enables quantitative detection of plasmin activity generated by Enterococcus faecalis-mediated activation of human glutamyl plasminogen in the presence of uPA, and the measured activity correlates with bacterial concentration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 569.62 (free base)
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Formula C27H38F3N5O5·xC2HF3O2
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Color White to off-white
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SMILES
O=C([C@H](N)C(C)C)N[C@H](C(N[C@H](C(NC1=CC=C(C(C(F)(F)F)=C2)C(OC2=O)=C1)=O)CCCCN)=O)CC(C)C.O=C(O)C(F)(F)F.[x]
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Synonyms
Plasmin substrate TFA
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Sequence
DVal-Leu-Lys-AFC
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Sequence Shortening
DVal-LK-AFC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)