2. Anti-infection
  3. HBV
  4. HBV-IN-54

HBV-IN-54 is a selective and orally active HBV inhibitor. HBV-IN-54 exhibits anti-HBV activity in both HepAD38 cells (EC50 = 0.020 μM, CC50 > 100 μM) and HLCZ01 cells (EC50 = 0.024 μM, CC50 > 100 μM). HBV-IN-54 inhibits viral replication in vivo. HBV-IN-54 displays favorable physicochemical properties and high plasma stability. HBV-IN-54 can be used for research on Hepatitis B (HBV).

For research use only. We do not sell to patients.

HBV-IN-54

HBV-IN-54 Chemical Structure

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HBV-IN-54 Related Antibodies

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Description

HBV-IN-54 is a selective and orally active HBV inhibitor. HBV-IN-54 exhibits anti-HBV activity in both HepAD38 cells (EC50 = 0.020 μM, CC50 > 100 μM) and HLCZ01 cells (EC50 = 0.024 μM, CC50 > 100 μM). HBV-IN-54 inhibits viral replication in vivo. HBV-IN-54 displays favorable physicochemical properties and high plasma stability. HBV-IN-54 can be used for research on Hepatitis B (HBV)[1].

In Vitro

HBV-IN-54 (compound 35a) binds stably to the HBV core protein, retaining hydrogen bonds with Trp-102, Thr-128, and Leu-140. HBV-IN-54 forms interactions with Ser-106, Thr-142, and Asn-136[1].
HBV-IN-54 (6 days) suppresses HBV DNA replication with EC50 of 0.02 μM, CC50 of > 100 μM and SI of > 5000 in the HepAD38 cell Line[1].
HBV-IN-54 (0.3-100 μM, 6 days) suppresses HBV DNA replication with an EC50 of approximately 0.024 μM in HLCZ01 cell Line[1].
HBV-IN-54 (1 mg/mL, 0-60 min) has stability in human liver microsomes with T1/2 = 30.8 min , CLint = 22.5 μL/min/mg protein, MPPGL = 48.8 mg protein/g tissue, OW = 25.7 g tissue/kg body weight, CLint, sce = 28.2 mL/min/kg[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HBV-IN-54 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: HLCZ01 Cells
Concentration: 0.3 μM, 10 μM, 30 μM, and 100 μM
Incubation Time: 3 days
Result: Exhibited minimal cytotoxicity at a concentration of 100 μM (inhibition rate 8.02%) and suppressed HBV DNA replication in a dose-dependent manner.
Parmacokinetics
Species Dose Route T1/2 C0 AUC0-t AUC0-∞ Vz CL MRT0-t MRT0-∞ Vss Tmax Cmax F
Rat 1 mg/mL i.v. 2.6 h 1005.53 ng/mL 766.2 ng·h/mL 772.84 ng·h/mL 4.88 L/kg 1.30 L/h/kg 1.09 h 1.21 h 1.56 L/kg / / /
Rat 5 mg/kg p.o. 1.69 h / 1847.17 ng·h/mL 1861.03 ng·h/mL 6.57 L/kg 2.69 L/h/kg 2.53 h 2.62 h / 1 h 560.33 ng/mL 48.22 %
In Vivo

HBV-IN-54 (compound 35a) (25 mg/kg, p.o., once daily, for 21 days) has anti-HBV activity in pAAV-HBV1.2 plasmid-induced male C57BL/6J mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: pAAV-HBV1.2 plasmid (6 μg, i.v., once)-induced male C57BL/6J mice (6 week-old)[1]
Dosage: 25 mg/kg
Administration: i.g., once daily for 21 days , blood samples were collected every 3 days
Result: Reduced serum HBV DNA levels by 1.88 log10 IU/mL.
Reduced the number of HBcAg-positive cells by 40%.
Did not induce obvious drug-induced liver injury.
Molecular Weight

448.53

Formula

C22H26F2N4O2S
SMILES

CC1=C(C=C(C(F)=C1)NC(N2CCN(CC2)CCO)=S)C(NC3=CC(C)=C(C=C3)F)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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HBV-IN-54 Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HBV-IN-54HBVHepatitis B virusanti-HBV activityHepAD38 cellsHepatitis BHLCZ01 cellsInhibitorinhibitorinhibit

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