Human IgG1 (K214R) kappa, Isotype Control 

Human IgG1 (K214R) kappa, Isotype Control is a human IgG1 kappa monoclonal antibody mutant with a lysine-to-arginine substitution at position 214. Human IgG1 (K214R) kappa, Isotype Control is mainly used as an isotype control in experiments such as immunoprecipitation. Human IgG1 (K214R) kappa, Isotype Control binds to proteins non-specifically, thereby helping researchers verify the interactions of specific antibodies and ensure the accuracy of experimental results^[1][2][3].

Human IgG1 (K214R) kappa, Isotype Control does not inhibit NTHi-induced MyD88 K63-linked polyubiquitination, and induces NF-κB promoter activity to a degree similar to that of wild-type MyD88^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

142.74 kDa

Liquid

Colorless to light yellow

[Human IgG1 (K214R) kappa, Isotype Control]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chen L, et al. SIAH1-mediated RPS3 ubiquitination contributes to chemosensitivity in epithelial ovarian cancer. Aging (Albany NY). 2022;14(15):6202-6226.

  [2]. Lee BC, et al. Deubiquitinase CYLD acts as a negative regulator for bacterium NTHi-induced inflammation by suppressing K63-linked ubiquitination of MyD88. Proc Natl Acad Sci U S A. 2016;113(2):E165-E171.

  [3]. Chen L, et al. Targeted destruction of VISTA boosts anti-tumor immunotherapy. Cell Res. 2025;35(12):987-1002.  [Content Brief]