1. Apoptosis
  2. Apoptosis
  3. HXR9 hydrochloride

HXR9 hydrochloride is a cell-permeable peptide and a competitive antagonist of HOX/PBX interaction. HXR9 hydrochloride antagonizes the interaction between HOX and a second transcrip-tion factor (PBX), which binds to HOX proteins in paralogue groups1 to 8. HXR9 hydrochloride selectively decreases cell proliferation and promotes apoptosis in cells with a high level of expression of the HOXA/PBX3 genes, such as MLL-rearranged leukemic cells.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

HXR9 hydrochloride Chemical Structure

HXR9 hydrochloride Chemical Structure

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1 mg USD 160 In-stock
5 mg USD 370 In-stock
10 mg USD 590 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of HXR9 hydrochloride:

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Description

HXR9 hydrochloride is a cell-permeable peptide and a competitive antagonist of HOX/PBX interaction. HXR9 hydrochloride antagonizes the interaction between HOX and a second transcrip-tion factor (PBX), which binds to HOX proteins in paralogue groups1 to 8. HXR9 hydrochloride selectively decreases cell proliferation and promotes apoptosis in cells with a high level of expression of the HOXA/PBX3 genes, such as MLL-rearranged leukemic cells[1][2][3].

In Vitro

HXR9 hydrochloride (60μM; 4 hours) blocks the interaction between PBX and HOX[1].
HXR9 hydrochloride (60μM; 2 hours) triggers apoptosis in B16 and primary melanoma cells[1].
HXR9 hydrochloride (60μM; 2 hours) causes specific transcriptional changes[1].
HXR9 hydrochloride (B16 cells) shows antiproliferative activity with an IC50 of 20μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line: murine B16melanoma cells
Concentration: 60 μM
Incubation Time: 4 hours
Result: Blocked the binding of HOXD9 to PBX.

Apoptosis Analysis

Cell Line: B16 cells
Concentration: 60 μM
Incubation Time: 2 hours
Result: A significant proportion of cells were in late phases of apoptosis.

RT-PCR

Cell Line: B16F10cells
Concentration: 60 μM
Incubation Time: 2 hours
Result: Fos, Jun, Dusp1, and Atf1,were allsignificantly up-regulate.
In Vivo

HXR9 hydrochloride (10 mg/kg; i.v. via the tail vein; twice weekly) blocks tumor growth[1].
HXR9 hydrochloride (Initial dose of 100 mg/kg (subsequent dosing of 10 mg/kg twice weekly); Intraperitoneal; twice weekly for 18 days) blocks A549 tumour growth in vivo[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57black/6 mice (bearing B16 cells)
Dosage: 10 mg/kg
Administration: I.v. via the tail vein; twice weekly (~30 days)
Result: Tumors showed a significant degree of growth retardation.
Animal Model: Athymic nude mice (bearing A549 cells)
Dosage: Initial dose of 100 mg/kg (subsequent dosing of 10 mg/kg twice weekly)
Administration: Intraperitoneal; twice weekly for 18 days
Result: The tumours of HXR9-treated mice were considerably smaller than those of the control groups.
Molecular Weight

2754.67

Formula

C119H194ClN53O20S

Appearance

Solid

Color

White to off-white

Sequence Shortening

WYPWMKKHHRRRRRRRRR

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (18.15 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3630 mL 1.8151 mL 3.6302 mL
5 mM 0.0726 mL 0.3630 mL 0.7260 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (36.30 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.3630 mL 1.8151 mL 3.6302 mL 9.0755 mL
5 mM 0.0726 mL 0.3630 mL 0.7260 mL 1.8151 mL
10 mM 0.0363 mL 0.1815 mL 0.3630 mL 0.9075 mL
15 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6050 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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HXR9 hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HXR9 hydrochloride
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HY-P3245A
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