DFHBI
Based on 7 publication(s) in Google Scholar
DFHBI is a small molecule that resembles the chromophore of green fluorescent protein (GFP). DFHBI can be used for imaging RNA in living cells. . Spinach and DFHBI are essentially nonfluorescent when unbound, whereas the Spinach-DFHBI complex is brightly fluorescent both in vitro and in living cells (Ex/Em = 469/501 nm).
For research use only. We do not sell to patients.
- Purity: 98.85%
- CAS No.: 1241390-29-3
- Formula: C12H10F2N2O2
- Molecular Weight:252.22
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Storage:
4°C, protect from light
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DFHBI
More- Nano Lett. 2022 Jan 26;22(2):716-725. [Abstract]
- Sens Actuators B Chem. 2024 Feb 20, 135521.
- Anal Chem. 2023 Sep 19;95(37):13762-13768. [Abstract]
- Anal Chim Acta. 2022 Jul 18:1217:340028. [Abstract]
- Anal Chim Acta. 2022 May 29;1209:339816. [Abstract]
- J Cell Biochem. 2024 Dec;125(12):e30249. [Abstract]
- Research Square Preprint. 2022 Jul.
Biological Activity
Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
1. Solution preparation[3]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 20 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 20 μM with PBS or cell culture medium (pH 7.4) (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 HEK293T cells are transfected with pAV-5S.
2.2 Add 20 μM DFHB into the cell plate.
2.3 Incubate the dye-loading plate in a cell incubator for 30 minutes.
2.4 Fluorescence microscopy detection (Ex/Em = 469/501 nm).
These RNAs interact with DFHBI to produce a bluish-green fluorescence emission (501 nm) after excitation at 447 nm. Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of fluorescent RNA. Spinach2 binds and activates the fluorescence of DFHBI, allowing the dynamic localizations of Spinach2-tagged RNAs to be imaged in live cells. The spectral properties of Spinach2 are limited by DFHBI, which produces fluorescence that is bluish-green and is not optimized for filters commonly used in fluorescence microscopes. Spinach and Spinach2 bind to DFHBI have fluorescence excitation maxima of 447 nm and peak fluorescence emission of 501 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1241390-29-3
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Appearance Solid
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Molecular Weight 252.22
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Formula C12H10F2N2O2
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Color Light yellow to yellow
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SMILES
O=C(N(C)C(C)=N/1)C1=C\C2=CC(F)=C(O)C(F)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
Publications (7)
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Journal Impact Factor
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Most Recent
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Nano Lett
The First FRET-Based RNA Aptamer NanoKit for Sensitively and Specifically Detecting c-di-GMP. [Abstract]2022 Jan 26;22(2):716-725. PMID: 34994567 -
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Anal Chem
Live-Cell Imaging of Endogenous RNA with a Genetically Encoded Fluorogenic Allosteric Aptamer. [Abstract]2023 Sep 19;95(37):13762-13768. PMID: 37661353 -
Anal Chim Acta
Target-triggered tertiary amplifications for sensitive and label-free protein detection based on lighting-up RNA aptamer transcriptions. [Abstract]2022 Jul 18:1217:340028. PMID: 35690424 -
Anal Chim Acta
2022 May 29;1209:339816. PMID: 35569846 -
J Cell Biochem
Trans-cleaving hammerhead ribozyme in specific regions can improve knockdown efficiency in vivo. [Abstract]2024 Dec;125(12):e30249. PMID: 35411616 -
Solvent & Solubility
DMSO : ≥ 83.33 mg/mL (330.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Song W, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. [Content Brief]
[2]. Strack RL, et al. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat Protoc. 2014 Jan;9(1):146-55. [Content Brief]
[3]. Paige JS, et al. RNA mimics of green fluorescent protein. Science. 2011 Jul 29;333(6042):642-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9648 mL | 19.8240 mL | 39.6479 mL | 99.1198 mL |
| 5 mM | 0.7930 mL | 3.9648 mL | 7.9296 mL | 19.8240 mL | |
| 10 mM | 0.3965 mL | 1.9824 mL | 3.9648 mL | 9.9120 mL | |
| 15 mM | 0.2643 mL | 1.3216 mL | 2.6432 mL | 6.6080 mL | |
| 20 mM | 0.1982 mL | 0.9912 mL | 1.9824 mL | 4.9560 mL | |
| 25 mM | 0.1586 mL | 0.7930 mL | 1.5859 mL | 3.9648 mL | |
| 30 mM | 0.1322 mL | 0.6608 mL | 1.3216 mL | 3.3040 mL | |
| 40 mM | 0.0991 mL | 0.4956 mL | 0.9912 mL | 2.4780 mL | |
| 50 mM | 0.0793 mL | 0.3965 mL | 0.7930 mL | 1.9824 mL | |
| 60 mM | 0.0661 mL | 0.3304 mL | 0.6608 mL | 1.6520 mL | |
| 80 mM | 0.0496 mL | 0.2478 mL | 0.4956 mL | 1.2390 mL | |
| 100 mM | 0.0396 mL | 0.1982 mL | 0.3965 mL | 0.9912 mL |
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