DFHO
Based on 1 publication(s) in Google Scholar
DFHO is a fluorogenic ligand of Corn fluorogenic aptamer. The RNA aptamer, Corn binds DFHO with a Kd value of 70 nM and converts it to a fluorescent form, enabling RNA imaging in cells.
For research use only. We do not sell to patients.
- Purity: 99.68%
- CAS No.: 1420815-34-4
- Formula: C12H9F2N3O3
- Molecular Weight:281.21
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DFHO
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Biological Activity
Kd: 70 nM (Corn binds DFHO)[1]
Corn is tested in E. coli. Minimal fluorescence that is seen in DFHO-treated cells (20 μM) transformed with the empty vector. However robust yellow fluorescence is seen in E. coli expressing Corn[1].
Corn-DFHO exhibits markedly enhanced photostability in vitro. The Corn-DFHO complexes are imaged in mammalian cells. Since the U6 promoter is one of the three major Pol III promoters, Corn from the U6 promoter is expressed and replaced the majority of the U6 sequence with Corn, leaving a minimal 5′ capping element leader sequence. The majority of the cells expressed diffuse yellow fluorescence, most prominently in the cytoplasm)[1].
DFHO exhibits low background fluorescence and cytotoxicity, making it a potentially suitable fluorophore for activation by RNA aptamers and for imaging experiments in living cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1420815-34-4
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Appearance Solid
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Molecular Weight 281.21
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Formula C12H9F2N3O3
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Color Yellow to brown
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SMILES
O=C(N1C)/C(N=C1/C=N/O)=C\C(C=C2F)=CC(F)=C2O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Res Sq
2025 Nov 4:rs.3.rs-7958216. PMID: 41282238
Solvent & Solubility
DMSO : ≥ 100 mg/mL (355.61 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Wenjiao Song, et al. Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat Chem Biol. 2017 Nov;13(11):1187-1194. [Content Brief]
[2]. Hyaeyeong Kim, et al. A Fluorogenic RNA-Based Sensor Activated by Metabolite-Induced RNA Dimerization. Cell Chem Biol. 2019 Dec 19;26(12):1725-1731.e6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5561 mL | 17.7803 mL | 35.5606 mL | 88.9015 mL |
| 5 mM | 0.7112 mL | 3.5561 mL | 7.1121 mL | 17.7803 mL | |
| 10 mM | 0.3556 mL | 1.7780 mL | 3.5561 mL | 8.8902 mL | |
| 15 mM | 0.2371 mL | 1.1854 mL | 2.3707 mL | 5.9268 mL | |
| 20 mM | 0.1778 mL | 0.8890 mL | 1.7780 mL | 4.4451 mL | |
| 25 mM | 0.1422 mL | 0.7112 mL | 1.4224 mL | 3.5561 mL | |
| 30 mM | 0.1185 mL | 0.5927 mL | 1.1854 mL | 2.9634 mL | |
| 40 mM | 0.0889 mL | 0.4445 mL | 0.8890 mL | 2.2225 mL | |
| 50 mM | 0.0711 mL | 0.3556 mL | 0.7112 mL | 1.7780 mL | |
| 60 mM | 0.0593 mL | 0.2963 mL | 0.5927 mL | 1.4817 mL | |
| 80 mM | 0.0445 mL | 0.2223 mL | 0.4445 mL | 1.1113 mL | |
| 100 mM | 0.0356 mL | 0.1778 mL | 0.3556 mL | 0.8890 mL |
DFHO Related Classifications
HY-136277 Related Classifications
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