CRANAD-2 

CRANAD-2 is a specific near-infrared (NIR) fluorescent probe for detecting Aβ plaques (unbound to Aβ: Ex=640 nm; Em=805 nm; after binding: Em=715 nm). CRANAD-2 penetrates the blood-brain barrier and has high affinity for Aβ aggregates, with a K_d value of 38 nM. CRANAD-2 is applicable to research related to Alzheimer's disease and neurodegenerative diseases^[1][2][3][4].

CRANAD-2 (0-1500 nM; 1 h) binds to preformed ΔN amyloid fibrils with a dissociation constant of 55 ± 6 nM^[1].
CRANAD-2 (35 nM) enables SMLM super-resolution imaging of preformed ΔN amyloid fibrils at a concentration of 35 nM, with a resolution of 45-55 nm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CRANAD-2 (2.0 mg/kg; i.v.; single dose) enables in vivo detection of Aβ deposits in the arcAβ mouse model via MSOT and epi-fluorescence imaging, with specific co-localization to Aβ deposits in brain sections^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

410.26

C₂₃H₂₅BF₂N₂O₂

1193447-34-5

Solid

Brown to black

CN(C)C(C=C1)=CC=C1/C=C/C2=O[B+3]([F-])([F-])O=C(/C=C/C3=CC=C(N(C)C)C=C3)[CH-]2

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Torra J, et al. Versatile Near-Infrared Super-Resolution Imaging of Amyloid Fibrils with the Fluorogenic Probe CRANAD-2. Chemistry. 2022;28(19):e202200026.  [Content Brief]

  [2]. Ni R, et al. In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice. Photoacoustics. 2021 Jul 15;23:100285.  [Content Brief]

  [3]. Ran C, et al. Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-beta deposits. J Am Chem Soc. 2009 Oct 28;131(42):15257-61.  [Content Brief]

  [4]. Ran C, et al. Non-conjugated small molecule FRET for differentiating monomers from higher molecular weight amyloid beta species. PLoS One. 2011 Apr 29;6(4):e19362.  [Content Brief]