DCP-Bio1
Based on 1 Customer Validation
DCP-Bio1 is a targeting labeling reagent for cysteine sulfenic acid (cysteine sulfenic acid). DCP-Bio1 labels protein cysteine sulfenic acid in intact cells via in situ labeling, and also labels cellular proteins during cell lysis. DCP-Bio1 detects sulfenic acid modifications of FLT3 and FLT3ITD in cancer cells. DCP-Bio1 is applicable to research related to sulfenic acid labeling
For research use only. We do not sell to patients.
- CAS No.: 882853-94-3
- Formula: C19H28N2O5S
- Molecular Weight:396.50
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
DCP-Bio1 (1 mM; variable times) incorporates into sulfenic acid-containing papaya papain, Escherichia coli C84,94S fRMsr, and Salmonella typhimurium C165S AhpC with distinct first-order rates of 1.65 min−1, 0.13 min−1, and 0.003 min−1, respectively[1].
DCP-Bio1 (0.1-5 mM) incorporation into sulfenic acid-containing proteins in HEK293 cell lysates increases with decreasing protein concentration and increasing DCP-Bio1 concentration, with no difference observed between tumor necrosis factor alpha-treated and untreated cells[1].
DCP-Bio1 (1 mM; 2 h) labels sulfenic acid residues on wild-type FLT3 in RS4-11 cells and FLT3ITD in MV4-11 and HEK293 cells, with cysteines 695 and 790 of FLT3ITD contributing significantly to the labeling signal[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 882853-94-3
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Appearance Solid
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Molecular Weight 396.50
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Formula C19H28N2O5S
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Color White to off-white
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SMILES
O=C(CC(CC1)=O)C1CCCOC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (252.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Klomsiri C, et al.. Use of dimedone-based chemical probes for sulfenic acid detection evaluation of conditions affecting probe incorporation into redox-sensitive proteins. Methods in enzymology. 2010;473:77-94. [Content Brief]
[2]. Böhmer A, et al.. Modulation of FLT3 signal transduction through cytoplasmic cysteine residues indicates the potential for redox regulation. Redox biology. 2020 Jan;28:101325. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5221 mL | 12.6103 mL | 25.2207 mL | 63.0517 mL |
| 5 mM | 0.5044 mL | 2.5221 mL | 5.0441 mL | 12.6103 mL | |
| 10 mM | 0.2522 mL | 1.2610 mL | 2.5221 mL | 6.3052 mL | |
| 15 mM | 0.1681 mL | 0.8407 mL | 1.6814 mL | 4.2034 mL | |
| 20 mM | 0.1261 mL | 0.6305 mL | 1.2610 mL | 3.1526 mL | |
| 25 mM | 0.1009 mL | 0.5044 mL | 1.0088 mL | 2.5221 mL | |
| 30 mM | 0.0841 mL | 0.4203 mL | 0.8407 mL | 2.1017 mL | |
| 40 mM | 0.0631 mL | 0.3153 mL | 0.6305 mL | 1.5763 mL | |
| 50 mM | 0.0504 mL | 0.2522 mL | 0.5044 mL | 1.2610 mL | |
| 60 mM | 0.0420 mL | 0.2102 mL | 0.4203 mL | 1.0509 mL | |
| 80 mM | 0.0315 mL | 0.1576 mL | 0.3153 mL | 0.7881 mL | |
| 100 mM | 0.0252 mL | 0.1261 mL | 0.2522 mL | 0.6305 mL |
DCP-Bio1 Related Classifications
HY-D3473 Related Classifications
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