Aβ aggregation-IN-1
Based on 1 Customer Validation
Aβ aggregation-IN-1 (Compound 1b) is a β-amyloid aggregation inhibitor/depolymerizer, with IC50 values of 3.92 μM and 7.19 μM, respectively. Aβ aggregation-IN-1 inhibits the activation of preformed β-amyloid fibrils, reactive oxygen species (ROS) and Caspase-3. Aβ aggregation-IN-1 can be used in research related to Alzheimer's disease.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.92%
- CAS 番号: 352525-91-8
- 分子式: C9H8BF3O2
- 分子量:215.97
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
Caspase アイソフォーム固有の製品をすべて表示
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生物活性
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Caspase 3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| SH-SY5Y | IC50 |
2.85 μM
Compound: 1b
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Neuroprotective activity in human APP695-transfected SH-SY5Y cells assessed as inhibition of H2O2-induced ROS production after 60 mins by DCFA-DA assay
Neuroprotective activity in human APP695-transfected SH-SY5Y cells assessed as inhibition of H2O2-induced ROS production after 60 mins by DCFA-DA assay
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[PMID: 23219701] |
Aβ aggregation-IN-1 (compound 1b) potently inhibits βA aggregation in a cell-free system with an IC50 of 3.92 μM[1].
Aβ aggregation-IN-1 (compound 1b) effectively disaggregates preformed βA fibrils in a cell-free system with an IC50 of 7.19 μM[1].
Aβ aggregation-IN-1 (compound 1b) potently inhibits hydrogen peroxide-induced ROS generation in APPswe neuroblastoma cells with an IC50 of 2.85 μM[1].
Aβ aggregation-IN-1 (compound 1b) reduces hydrogen peroxide-induced caspase-3 activation by over 50% in APPswe neuroblastoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 352525-91-8
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性状 Solid
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分子量 215.97
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分子式 C9H8BF3O2
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Color White to off-white
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SMILES
FC(F)(F)C1=CC=C(/C=C/B(O)O)C=C1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (463.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (271 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.6303 mL | 23.1514 mL | 46.3027 mL | 115.7568 mL |
| 5 mM | 0.9261 mL | 4.6303 mL | 9.2605 mL | 23.1514 mL | |
| 10 mM | 0.4630 mL | 2.3151 mL | 4.6303 mL | 11.5757 mL | |
| 15 mM | 0.3087 mL | 1.5434 mL | 3.0868 mL | 7.7171 mL | |
| 20 mM | 0.2315 mL | 1.1576 mL | 2.3151 mL | 5.7878 mL | |
| 25 mM | 0.1852 mL | 0.9261 mL | 1.8521 mL | 4.6303 mL | |
| 30 mM | 0.1543 mL | 0.7717 mL | 1.5434 mL | 3.8586 mL | |
| 40 mM | 0.1158 mL | 0.5788 mL | 1.1576 mL | 2.8939 mL | |
| 50 mM | 0.0926 mL | 0.4630 mL | 0.9261 mL | 2.3151 mL | |
| 60 mM | 0.0772 mL | 0.3859 mL | 0.7717 mL | 1.9293 mL | |
| 80 mM | 0.0579 mL | 0.2894 mL | 0.5788 mL | 1.4470 mL | |
| 100 mM | 0.0463 mL | 0.2315 mL | 0.4630 mL | 1.1576 mL |
Aβ aggregation-IN-1 関連分類
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