DFHBI-2T
Based on 1 Customer Validation
DFHBI-2T is a membrane-permeable, RNA aptamer-activated fluorescent probe (Ex/Em=500 nm/523 nm), with a Kd value of 1300 nM for the RNA aptamer Spinach2. DFHBI-2T binds to Spinach2, converting the fluorophore to a fluorescent state, stabilizing its planar structure to facilitate the radiative fluorescence decay pathway, and forming a complex with red-shifted excitation and emission peaks. DFHBI-2T can be used for RNA imaging in live cells. It is also applicable to research related to fragile X-associated tremor/ataxia syndrome.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.0%
- CAS 番号: 1539318-40-5
- 分子式: C12H7F5N2O2
- 分子量:306.19
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
DFHBI-2T (1 μM) binds Spinach2 RNA aptamer with a Kd of 1300 nM, forming a complex with excitation maximum at 500 nm, emission maximum at 523 nm[1].
DFHBI-2T (20 μM; 10 min) forms a fluorescent complex that is readily detectable using a YFP filter cube but not a GFP filter cube in COS7 cells expressing (CGG)60-Spinach2[1].
DFHBI-2T enables SAM-dependent fluorescence detection at 523 nm using a Spinach2-based SAM sensor in vitro[1].
DFHBI-2T binds to the Spinach2 RNA aptamer with a Kd of 1300 nM, exhibiting a shift in spectral properties and a 60-fold increase in brightness compared to its free state[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1539318-40-5
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性状 Solid
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分子量 306.19
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分子式 C12H7F5N2O2
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Color Light yellow to yellow
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SMILES
O=C1N(C)C(C(F)(F)F)=N/C1=C\C2=CC(F)=C(O)C(F)=C2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (326.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2659 mL | 16.3297 mL | 32.6595 mL | 81.6486 mL |
| 5 mM | 0.6532 mL | 3.2659 mL | 6.5319 mL | 16.3297 mL | |
| 10 mM | 0.3266 mL | 1.6330 mL | 3.2659 mL | 8.1649 mL | |
| 15 mM | 0.2177 mL | 1.0886 mL | 2.1773 mL | 5.4432 mL | |
| 20 mM | 0.1633 mL | 0.8165 mL | 1.6330 mL | 4.0824 mL | |
| 25 mM | 0.1306 mL | 0.6532 mL | 1.3064 mL | 3.2659 mL | |
| 30 mM | 0.1089 mL | 0.5443 mL | 1.0886 mL | 2.7216 mL | |
| 40 mM | 0.0816 mL | 0.4082 mL | 0.8165 mL | 2.0412 mL | |
| 50 mM | 0.0653 mL | 0.3266 mL | 0.6532 mL | 1.6330 mL | |
| 60 mM | 0.0544 mL | 0.2722 mL | 0.5443 mL | 1.3608 mL | |
| 80 mM | 0.0408 mL | 0.2041 mL | 0.4082 mL | 1.0206 mL | |
| 100 mM | 0.0327 mL | 0.1633 mL | 0.3266 mL | 0.8165 mL |
DFHBI-2T 関連分類
HY-110251A 関連分類
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