1. Apoptosis
  2. Caspase
    Apoptosis
  3. KEA1-97

KEA1-97 

Cat. No.: HY-114982 Purity: 99.66%
Handling Instructions

KEA1-97 is a selective Thioredoxin-caspase 3 interaction disruptor (IC50=10 μM). KEA1-97 disrupts the interaction of thioredoxin with caspase 3, activates caspases, and induces apoptosis without affecting thioredoxin activity.

For research use only. We do not sell to patients.

KEA1-97 Chemical Structure

KEA1-97 Chemical Structure

CAS No. : 2138882-71-8

Size Stock
Solution
10 mM * 1 mL in DMSO Check price and availability
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
Check price and availability
Solid
5 mg Check price and availability
10 mg Check price and availability
25 mg Check price and availability
50 mg Check price and availability
100 mg Check price and availability

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

KEA1-97 is a selective Thioredoxin-caspase 3 interaction disruptor (IC50=10 μM). KEA1-97 disrupts the interaction of thioredoxin with caspase 3, activates caspases, and induces apoptosis without affecting thioredoxin activity[1].

IC50 & Target[1]

Caspase 3

10 μM (IC50)

Thioredoxin

10 μM (IC50)

In Vitro

KEA1-97 (100 μM; 231MFP cells) impaires thioredoxin pulldown of caspase 3[1].
KEA1-97 (10 μM; 48 hours; 231MFP cells) impaires 231MFP serum-free cell proliferation[1].
KEA1-97 (10 μM; 0~12 hours; 231MFP cells) activates caspase 3/7 and induces apoptotic cell death[1].
KEA1-97 (231MFP cells) resistants to survival and proliferation impairments[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: 231MFP cells
Concentration: 100 μM
Incubation Time:
Result: Impaired thioredoxin pulldown of caspase 3.

Cell Proliferation Assay[1]

Cell Line: 231MFP cells
Concentration: 10 μM
Incubation Time: 48 h
Result: Impaired 231MFP serum-free cell proliferation.

Apoptosis Analysis[1]

Cell Line: 231MFP cells
Concentration: 10 μM
Incubation Time: 0~12 h
Result: Activated caspase 3/7 and induced apoptotic cell death.
In Vivo

KEA1-97 (5 mg/kg; i.p.; 50 days) attenuates tumor xenograft growth[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Immune-deficient SCID mice
Dosage: 5 mg/kg
Administration: I.p.; 50 days
Result: Attenuated tumor xenograft growth.
Molecular Weight

335.16

Formula

C₁₅H₉Cl₂FN₄

CAS No.
SMILES

ClC1=NC(Cl)=NC(NC2=CC=C(C=C2)C3=CC=C(C=C3)F)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (298.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9836 mL 14.9182 mL 29.8365 mL
5 mM 0.5967 mL 2.9836 mL 5.9673 mL
10 mM 0.2984 mL 1.4918 mL 2.9836 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

KEA1-97CaspaseApoptosis231MFPSelectiveDCTalkyneApoptoticProliferationInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
KEA1-97
Cat. No.:
HY-114982
Quantity:
MCE Japan Authorized Agent: