KEA1-97
Based on 1 Customer Validation
KEA1-97 is a selective Thioredoxin-caspase 3 interaction disruptor (IC50=10 μM). KEA1-97 disrupts the interaction of thioredoxin with caspase 3, activates caspases, and induces apoptosis without affecting thioredoxin activity.
For research use only. We do not sell to patients.
- Purity: 98.05%
- CAS No.: 2138882-71-8
- Formula: C15H9Cl2FN4
- Molecular Weight:335.16
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Apoptosis Compound Library
HY-L003
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Anti-Cancer Compound Library
HY-L025
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Covalent Screening Library
HY-L036
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Pyroptosis Compound Library
HY-L059
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Neurodegenerative Disease-related Compound Library
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Targeted Diversity Library
HY-L099
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Protease Inhibitor Library
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Highly Selective Inhibitors Library
HY-L158
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Highly Selective Activators Library
HY-L159
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Cell Death Library
HY-L162
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Multi-Target Compound Library
HY-L176
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MCE Bioactive Compound Library
HY-L001V
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Covalent Screening Library Plus
HY-L036P
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
All Caspase Isoforms
More
Biological Activity
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Caspase 3 10 μM (IC50) |
Thioredoxin 10 μM (IC50) |
KEA1-97 (100 μM; 231MFP cells) impaires thioredoxin pulldown of caspase 3[1].
KEA1-97 (10 μM; 48 hours; 231MFP cells) impaires 231MFP serum-free cell proliferation[1].
KEA1-97 (10 μM; 0~12 hours; 231MFP cells) activates caspase 3/7 and induces apoptotic cell death[1].
KEA1-97 (231MFP cells) resistants to survival and proliferation impairments[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:231MFP cells
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Concentration:100 μM
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Incubation Time:
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Result:Impaired thioredoxin pulldown of caspase 3.
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Cell Line:231MFP cells
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Concentration:10 μM
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Incubation Time:48 h
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Result:Impaired 231MFP serum-free cell proliferation.
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Cell Line:231MFP cells
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Concentration:10 μM
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Incubation Time:0~12 h
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Result:Activated caspase 3/7 and induced apoptotic cell death.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Immune-deficient SCID mice
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Dosage:5 mg/kg
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Administration:I.p.; 50 days
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Result:Attenuated tumor xenograft growth.
Chemical Information
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CAS No. 2138882-71-8
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Appearance Solid
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Molecular Weight 335.16
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Formula C15H9Cl2FN4
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Color White to light yellow
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SMILES
ClC1=NC(Cl)=NC(NC2=CC=C(C=C2)C3=CC=C(C=C3)F)=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (298.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9836 mL | 14.9182 mL | 29.8365 mL | 74.5912 mL |
| 5 mM | 0.5967 mL | 2.9836 mL | 5.9673 mL | 14.9182 mL | |
| 10 mM | 0.2984 mL | 1.4918 mL | 2.9836 mL | 7.4591 mL | |
| 15 mM | 0.1989 mL | 0.9945 mL | 1.9891 mL | 4.9727 mL | |
| 20 mM | 0.1492 mL | 0.7459 mL | 1.4918 mL | 3.7296 mL | |
| 25 mM | 0.1193 mL | 0.5967 mL | 1.1935 mL | 2.9836 mL | |
| 30 mM | 0.0995 mL | 0.4973 mL | 0.9945 mL | 2.4864 mL | |
| 40 mM | 0.0746 mL | 0.3730 mL | 0.7459 mL | 1.8648 mL | |
| 50 mM | 0.0597 mL | 0.2984 mL | 0.5967 mL | 1.4918 mL | |
| 60 mM | 0.0497 mL | 0.2486 mL | 0.4973 mL | 1.2432 mL | |
| 80 mM | 0.0373 mL | 0.1865 mL | 0.3730 mL | 0.9324 mL | |
| 100 mM | 0.0298 mL | 0.1492 mL | 0.2984 mL | 0.7459 mL |
KEA1-97 Related Classifications
HY-114982 Related Classifications
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