2. Apoptosis
  3. Caspase Apoptosis
  4. KEA1-97

KEA1-97 is a selective Thioredoxin-caspase 3 interaction disruptor (IC50=10 μM). KEA1-97 disrupts the interaction of thioredoxin with caspase 3, activates caspases, and induces apoptosis without affecting thioredoxin activity.

For research use only. We do not sell to patients.

KEA1-97 Chemical Structure

KEA1-97 Chemical Structure

CAS No. : 2138882-71-8

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solution
10 mM * 1 mL in DMSO USD 77 In-stock
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10 mg USD 110 In-stock
50 mg USD 380 In-stock
100 mg USD 610 In-stock
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Based on 1 publication(s) in Google Scholar

KEA1-97 Related Antibodies

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

KEA1-97 is a selective Thioredoxin-caspase 3 interaction disruptor (IC50=10 μM). KEA1-97 disrupts the interaction of thioredoxin with caspase 3, activates caspases, and induces apoptosis without affecting thioredoxin activity[1].

IC50 & Target[1]

Caspase 3

10 μM (IC50)

Thioredoxin

10 μM (IC50)

In Vitro

KEA1-97 (100 μM; 231MFP cells) impaires thioredoxin pulldown of caspase 3[1].
KEA1-97 (10 μM; 48 hours; 231MFP cells) impaires 231MFP serum-free cell proliferation[1].
KEA1-97 (10 μM; 0~12 hours; 231MFP cells) activates caspase 3/7 and induces apoptotic cell death[1].
KEA1-97 (231MFP cells) resistants to survival and proliferation impairments[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KEA1-97 Related Antibodies

Western Blot Analysis[1]

Cell Line: 231MFP cells
Concentration: 100 μM
Incubation Time:
Result: Impaired thioredoxin pulldown of caspase 3.

Cell Proliferation Assay[1]

Cell Line: 231MFP cells
Concentration: 10 μM
Incubation Time: 48 h
Result: Impaired 231MFP serum-free cell proliferation.

Apoptosis Analysis[1]

Cell Line: 231MFP cells
Concentration: 10 μM
Incubation Time: 0~12 h
Result: Activated caspase 3/7 and induced apoptotic cell death.
In Vivo

KEA1-97 (5 mg/kg; i.p.; 50 days) attenuates tumor xenograft growth[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Immune-deficient SCID mice
Dosage: 5 mg/kg
Administration: I.p.; 50 days
Result: Attenuated tumor xenograft growth.
Molecular Weight

335.16

Formula

C15H9Cl2FN4
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

ClC1=NC(Cl)=NC(NC2=CC=C(C=C2)C3=CC=C(C=C3)F)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (298.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9836 mL 14.9182 mL 29.8365 mL
5 mM 0.5967 mL 2.9836 mL 5.9673 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.66%

COA (245 KB) HNMR (248 KB) LCMS (99 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9836 mL 14.9182 mL 29.8365 mL 74.5912 mL
5 mM 0.5967 mL 2.9836 mL 5.9673 mL 14.9182 mL
10 mM 0.2984 mL 1.4918 mL 2.9836 mL 7.4591 mL
15 mM 0.1989 mL 0.9945 mL 1.9891 mL 4.9727 mL
20 mM 0.1492 mL 0.7459 mL 1.4918 mL 3.7296 mL
25 mM 0.1193 mL 0.5967 mL 1.1935 mL 2.9836 mL
30 mM 0.0995 mL 0.4973 mL 0.9945 mL 2.4864 mL
40 mM 0.0746 mL 0.3730 mL 0.7459 mL 1.8648 mL
50 mM 0.0597 mL 0.2984 mL 0.5967 mL 1.4918 mL
60 mM 0.0497 mL 0.2486 mL 0.4973 mL 1.2432 mL
80 mM 0.0373 mL 0.1865 mL 0.3730 mL 0.9324 mL
100 mM 0.0298 mL 0.1492 mL 0.2984 mL 0.7459 mL
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KEA1-97 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KEA1-972138882-71-8CaspaseApoptosis231MFPSelectiveDCTalkyneApoptoticProliferationInhibitorinhibitorinhibit

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