Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate
Based on 1 Customer Validation
Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate, a natural coumarins derivative, is exploited for the synthesis of the switchable fluorescent substrates to be used in bacterial enzyme detection.
For research use only. We do not sell to patients.
- Purity : 97.39%
- CAS No.: 15991-13-6
- Formula: C12H10O5
- Molecular Weight:234.20
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (compound 2) exhibits the maximum absorption (λabs~322 nm) and emission (λem~387 nm) wavelengths[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 15991-13-6
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Appearance Solid
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Molecular Weight 234.20
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Formula C12H10O5
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Color White to light yellow
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SMILES
O=C1OC(C=C(O)C=C2)=C2C(CC(OC)=O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 116.67 mg/mL (498.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Nascimento JSD, et al. Mapping the Biotransformation of Coumarins through Filamentous Fungi. Molecules. 2019 Sep 29;24(19). [Content Brief]
[2]. Lizzul-Jurse A, et al. Readily functionalizable phosphonium-tagged fluorescent coumarins for enhanced detection of conjugates by mass spectrometry. Org Biomol Chem. 2016 Aug 10;14(32):7777-91. [Content Brief]
[3]. Giovannini G, et al. Coumarin-based, switchable fluorescent substrates for enzymatic bacterial detection. Talanta. 2018 Oct 1;188:448-453. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.2699 mL | 21.3493 mL | 42.6985 mL | 106.7464 mL |
| 5 mM | 0.8540 mL | 4.2699 mL | 8.5397 mL | 21.3493 mL | |
| 10 mM | 0.4270 mL | 2.1349 mL | 4.2699 mL | 10.6746 mL | |
| 15 mM | 0.2847 mL | 1.4233 mL | 2.8466 mL | 7.1164 mL | |
| 20 mM | 0.2135 mL | 1.0675 mL | 2.1349 mL | 5.3373 mL | |
| 25 mM | 0.1708 mL | 0.8540 mL | 1.7079 mL | 4.2699 mL | |
| 30 mM | 0.1423 mL | 0.7116 mL | 1.4233 mL | 3.5582 mL | |
| 40 mM | 0.1067 mL | 0.5337 mL | 1.0675 mL | 2.6687 mL | |
| 50 mM | 0.0854 mL | 0.4270 mL | 0.8540 mL | 2.1349 mL | |
| 60 mM | 0.0712 mL | 0.3558 mL | 0.7116 mL | 1.7791 mL | |
| 80 mM | 0.0534 mL | 0.2669 mL | 0.5337 mL | 1.3343 mL | |
| 100 mM | 0.0427 mL | 0.2135 mL | 0.4270 mL | 1.0675 mL |
Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate Related Classifications
HY-W030319 Related Classifications
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