2. Metabolic Enzyme/Protease Cytoskeleton
  3. MMP Cadherin
  4. Murine Fibrinogen

Murine Fibrinogen is a native fibrinogen derived from mouse plasma. Murine Fibrinogen acts as a cerebrovascular permeability enhancer. Murine Fibrinogen activates matrix metalloproteinase-9 (MMP-9), downregulates the expression of vascular endothelial cadherin (VE-cadherin), and upregulates the expression of plasmalemmal vesicle-associated protein-1 (PV-1). Murine Fibrinogen increases macromolecular leakage from pial veins, thereby disrupting the microvascular integrity of cerebral blood vessels. Murine Fibrinogen can be used in studies related to cerebrovascular dysfunction.

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Murine Fibrinogen

Murine Fibrinogen Chemical Structure

CAS No. : 9001-32-5

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Murine Fibrinogen Related Antibodies

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Description

Murine Fibrinogen is a native fibrinogen derived from mouse plasma. Murine Fibrinogen acts as a cerebrovascular permeability enhancer. Murine Fibrinogen activates matrix metalloproteinase-9 (MMP-9), downregulates the expression of vascular endothelial cadherin (VE-cadherin), and upregulates the expression of plasmalemmal vesicle-associated protein-1 (PV-1). Murine Fibrinogen increases macromolecular leakage from pial veins, thereby disrupting the microvascular integrity of cerebral blood vessels. Murine Fibrinogen can be used in studies related to cerebrovascular dysfunction[1].

IC50 & Target[1]

MMP-9

 

In Vivo

Murine fibrinogen (20 mg per 100 g of body weight; carotid artery infusion; 20 mL/min; 10 minutes) induces significant pial venular macromolecular leakage, ICAM-1 and PV-1 upregulation, VE-cadherin downregulation, and MMP activation in mice, with reduced effects in MMP9-/- mice, indicating MMP-9 mediates part of the reagent's cerebrovascular dysfunction effects[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (12-week-old male, 26-30 g, wild-type); MMP9-/- (FVB.Cg-Mmp9tm1Tvu/J, 12-week-old male, 26-30 g, MMP-9 gene knockout); FVB/NJ (12-week-old male, 26-30 g, wild-type)[1]
Dosage: 20 mg per 100 g of body weight (resulting in a blood concentration of 4 mg/mL)
Administration: carotid artery infusion; 20 mL/min; 10 minutes
Result: Induced significantly greater pial venular macromolecular leakage (fluorescence intensity of 155 FIU) than PBS infusion in wild-type C57BL/6J mice.
Increased endothelial ICAM-1 expression to 50 FIU (vs 22 FIU with PBS) in wild-type C57BL/6J mice.
Reduced VE-cadherin expression to 22 FIU (vs 43 FIU with PBS) in wild-type C57BL/6J mice.
Increased MMP activity to 71 FIU in wild-type C57BL/6J mice.
Increased PV-1 expression to 170 FIU (vs 50 FIU with PBS) in wild-type C57BL/6J mice.
Induced further increased pial venular leakage (fluorescence intensity of 182 FIU) when combined with topical histamine in wild-type C57BL/6J mice.
Induced significantly greater pial venular macromolecular leakage (fluorescence intensity of 137 FIU) than PBS infusion in MMP9-/- mice, but less leakage than in wild-type mice.
Increased endothelial ICAM-1 expression to 39 FIU (vs 24 FIU with PBS) in MMP9-/- mice.
Reduced VE-cadherin expression to 28 FIU (vs 78 FIU with PBS) in MMP9-/- mice, with higher VE-cadherin levels than in Fg-treated wild-type mice.
Increased MMP activity to 46 FIU in MMP9-/- mice, with lower activity than in Fg-treated wild-type mice.
Increased PV-1 expression to 75 FIU (vs 50 FIU with PBS) in MMP9-/- mice, with lower expression than in Fg-treated wild-type mice.
Induced further increased pial venular leakage (fluorescence intensity of 152 FIU) when combined with topical histamine in MMP9-/- mice, but less leakage than in histamine-exposed, Fg-treated wild-type mice.
Induced pial venular macromolecular leakage (fluorescence intensity of 152 FIU) comparable to that in Fg-treated wild-type mice in FVB/NJ mice, with significantly greater leakage than PBS infusion.
Induced further increased pial venular leakage (fluorescence intensity of 178 FIU) when combined with topical histamine in FVB/NJ mice, comparable to that in histamine-exposed, Fg-treated wild-type mice.
Showed no degradation post-infusion.
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Murine Fibrinogen]
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

SDS (251 KB)

COA (242 KB)

Handling Instructions (2659 KB)
References
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Murine Fibrinogen Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Murine Fibrinogen9001-32-5MMPCadherinMatrix metalloproteinasesMMP-9vascular endothelial cadherinplasmalemmal vesicle-associated protein-1PV-1intercellular adhesion molecule-1cerebrovascular dysfunctionmatrix metalloproteinase-9ICAM-1VE-cadherinInhibitorinhibitorinhibit

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