Murine Fibrinogen

C57BL/6J (12-week-old male, 26-30 g, wild-type); MMP9-/- (FVB.Cg-Mmp9tm1Tvu/J, 12-week-old male, 26-30 g, MMP-9 gene knockout); FVB/NJ (12-week-old male, 26-30 g, wild-type)^[1]

20 mg per 100 g of body weight (resulting in a blood concentration of 4 mg/mL)

carotid artery infusion; 20 mL/min; 10 minutes

Induced significantly greater pial venular macromolecular leakage (fluorescence intensity of 155 FIU) than PBS infusion in wild-type C57BL/6J mice.
Increased endothelial ICAM-1 expression to 50 FIU (vs 22 FIU with PBS) in wild-type C57BL/6J mice.
Reduced VE-cadherin expression to 22 FIU (vs 43 FIU with PBS) in wild-type C57BL/6J mice.
Increased MMP activity to 71 FIU in wild-type C57BL/6J mice.
Increased PV-1 expression to 170 FIU (vs 50 FIU with PBS) in wild-type C57BL/6J mice.
Induced further increased pial venular leakage (fluorescence intensity of 182 FIU) when combined with topical histamine in wild-type C57BL/6J mice.
Induced significantly greater pial venular macromolecular leakage (fluorescence intensity of 137 FIU) than PBS infusion in MMP9-/- mice, but less leakage than in wild-type mice.
Increased endothelial ICAM-1 expression to 39 FIU (vs 24 FIU with PBS) in MMP9-/- mice.
Reduced VE-cadherin expression to 28 FIU (vs 78 FIU with PBS) in MMP9-/- mice, with higher VE-cadherin levels than in Fg-treated wild-type mice.
Increased MMP activity to 46 FIU in MMP9-/- mice, with lower activity than in Fg-treated wild-type mice.
Increased PV-1 expression to 75 FIU (vs 50 FIU with PBS) in MMP9-/- mice, with lower expression than in Fg-treated wild-type mice.
Induced further increased pial venular leakage (fluorescence intensity of 152 FIU) when combined with topical histamine in MMP9-/- mice, but less leakage than in histamine-exposed, Fg-treated wild-type mice.
Induced pial venular macromolecular leakage (fluorescence intensity of 152 FIU) comparable to that in Fg-treated wild-type mice in FVB/NJ mice, with significantly greater leakage than PBS infusion.
Induced further increased pial venular leakage (fluorescence intensity of 178 FIU) when combined with topical histamine in FVB/NJ mice, comparable to that in histamine-exposed, Fg-treated wild-type mice.
Showed no degradation post-infusion.