Neutrophil elastase inhibitor 3
Based on 1 Customer Validation
Neutrophil elastase inhibitor 3 (compound 13), a benzoxazinone analog, shows effects on superoxide anion generation and exhibits as a neutrophil elastase (NE) inhibitor, with an IC50 of 80.8 nM for NE release.
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- Pureza: 97.23%
- No. CAS: 1234707-32-4
- Fòrmula: C14H7BrClNO2
- Peso molecular:336.57
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
80.8 nM
Compound: 13
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Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP-induced neutrophil elastase release treated 5 mins before FMLP challenge measured after 10 mins
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP-induced neutrophil elastase release treated 5 mins before FMLP challenge measured after 10 mins
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[PMID: 20417587] |
| Neutrophil | IC50 |
25 μM
Compound: 13
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Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP-induced superoxide anion generation treated 5 mins before FMLP challenge measured after 10 mins by spectrophotometer analysis
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP-induced superoxide anion generation treated 5 mins before FMLP challenge measured after 10 mins by spectrophotometer analysis
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[PMID: 20417587] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1234707-32-4
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Appearance Solid
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Peso molecular 336.57
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Fòrmula C14H7BrClNO2
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Color White to light yellow
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SMILES
O=C1OC(C2=CC=CC=C2Br)=NC3=CC=CC(Cl)=C13
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (297.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9712 mL | 14.8558 mL | 29.7115 mL | 74.2788 mL |
| 5 mM | 0.5942 mL | 2.9712 mL | 5.9423 mL | 14.8558 mL | |
| 10 mM | 0.2971 mL | 1.4856 mL | 2.9712 mL | 7.4279 mL | |
| 15 mM | 0.1981 mL | 0.9904 mL | 1.9808 mL | 4.9519 mL | |
| 20 mM | 0.1486 mL | 0.7428 mL | 1.4856 mL | 3.7139 mL | |
| 25 mM | 0.1188 mL | 0.5942 mL | 1.1885 mL | 2.9712 mL | |
| 30 mM | 0.0990 mL | 0.4952 mL | 0.9904 mL | 2.4760 mL | |
| 40 mM | 0.0743 mL | 0.3714 mL | 0.7428 mL | 1.8570 mL | |
| 50 mM | 0.0594 mL | 0.2971 mL | 0.5942 mL | 1.4856 mL | |
| 60 mM | 0.0495 mL | 0.2476 mL | 0.4952 mL | 1.2380 mL | |
| 80 mM | 0.0371 mL | 0.1857 mL | 0.3714 mL | 0.9285 mL | |
| 100 mM | 0.0297 mL | 0.1486 mL | 0.2971 mL | 0.7428 mL |
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