NF-κB-IN-8
NF-κB-IN-8 competitively antagonizes LPS binding to MD-2. NF-κB-IN-8 reduces the expression of inflammatory factors by binding to MD-2. NF-κB-IN-8 also inhibits ALP activity. NF-κB-IN-8 can be used for the research of inflammation such as acute lung injury (ALI).
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- CAS. Nr.: 2924565-59-1
- Formel: C24H21N3O3
- Molecular Weight:399.44
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
2.58 μM
Compound: L26
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Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as decrease in LPS-induced IL-6 secretion pretreated for 30 mins followed by LPS-induction and measured after 24 hrs by ELISA analysis
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as decrease in LPS-induced IL-6 secretion pretreated for 30 mins followed by LPS-induction and measured after 24 hrs by ELISA analysis
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[PMID: 36963290] |
| RAW264.7 | IC50 |
8.41 μM
Compound: L26
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Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as decrease in LPS-induced TNF-alpha secretion pretreated for 30 mins followed by LPS-induction and measured after 24 hrs by ELISA analysis
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as decrease in LPS-induced TNF-alpha secretion pretreated for 30 mins followed by LPS-induction and measured after 24 hrs by ELISA analysis
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[PMID: 36963290] |
In Vitro
NF-κB-IN-8 (Compound L26) (10 μM) inhibits ALP activity by 64.30%[1].
NF-κB-IN-8 (1-10 μM, 24 h) inhibits expression of IL-6 and TNF-α in RAW 264.7 cells[1].
NF-κB-IN-8 (0-50 μM) inhibits the binding of LPS to MD-2, determined by ELISA Asssay[1].
NF-κB-IN-8 (50 μM, overnight) inhibits the formation of LPS/MD-2/TLR4 polymers in RAW264.7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7 cells
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Concentration:50 μM
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Incubation Time:Overnight
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Result:Inhibited the expression of LPS/MD-2/TLR4 polymers.
In Vivo
NF-κB-IN-8 (1000 and 1500 mg/kg, intra-gastrically administration) is low in toxic and safe in mice[1].
NF-κB-IN-8 (10 mg/kg, rats) shows T1/2: 4.2 h, Cmax: 163.288 μg/L[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ALI mice induced by LPS[1]
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Dosage:5 mg/kg
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Administration:Intra-gastrically administration
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Result:Downregulated the expression of pro-inflammatory factor Mrna (TNF-α).
Decreased ALP level in bronchoalveolar lavage fluid.
Inhibited the phosphorylation of p65 and increased the release of IκB-α. (WB assay, lung tissue sample)
Chemical Information
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CAS. Nr. 2924565-59-1
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Molecular Weight 399.44
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Formel C24H21N3O3
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SMILES
COC1=CC=CC(COC2=CC=C(C=C2)/C=C/C(NC3=NC4=C(N3)C=CC=C4)=O)=C1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
NF-κB-IN-8 Related Classifications
HY-149248 Related Classifications
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