Cat. No.: HY-119198

NSC745885

Customer Review

Based on 1 Customer Validation

NSC745885 an effective anti-tumor agent, shows selective toxicity against multiple cancer cell lines but not normal cells. NSC745885 is an effective down-regulator of EZH2 via proteasome-mediated degradation. NSC745885 provides possibilities for the study of advanced bladder and oral squamous cell carcinoma (OSCC) cancers.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 4219-52-7
Formula: C₁₄H₆N₂O₂S
Molecular Weight:266.27

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

NSC745885 Chemical Structure

CAS No.: 4219-52-7

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]

EZH2

All Histone Methyltransferase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	GI₅₀	6.17 μM Compound: 23, NSC-745885	Growth inhibition of human 786-0 cells Growth inhibition of human 786-0 cells	[PMID: 19804981]
A498	GI₅₀	12.4 μM Compound: 23, NSC-745885	Growth inhibition of human A498 cells Growth inhibition of human A498 cells	[PMID: 19804981]
A549	GI₅₀	14.7 μM Compound: 23, NSC-745885	Growth inhibition of human A549 cells Growth inhibition of human A549 cells	[PMID: 19804981]
ACHN	GI₅₀	1.46 μM Compound: 23, NSC-745885	Growth inhibition of human ACHN cells Growth inhibition of human ACHN cells	[PMID: 19804981]
BT-549	GI₅₀	1.63 μM Compound: 23, NSC-745885	Growth inhibition of human BT549 cells Growth inhibition of human BT549 cells	[PMID: 19804981]
CAKI-1	GI₅₀	1.61 μM Compound: 23, NSC-745885	Growth inhibition of human Caki1 cells Growth inhibition of human Caki1 cells	[PMID: 19804981]
CCRF-CEM	GI₅₀	1.08 μM Compound: 23, NSC-745885	Growth inhibition of human CCRF-CEM cells Growth inhibition of human CCRF-CEM cells	[PMID: 19804981]
COLO 205	GI₅₀	13.4 μM Compound: 23, NSC-745885	Growth inhibition of human COLO205 cells Growth inhibition of human COLO205 cells	[PMID: 19804981]
DU-145	GI₅₀	1.67 μM Compound: 23, NSC-745885	Growth inhibition of human DU145 cells Growth inhibition of human DU145 cells	[PMID: 19804981]
DU-145	IC₅₀	7.41 μM Compound: 2, NSC745885	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay Cytotoxicity against human DU145 cells after 72 hrs by MTT assay	[PMID: 26344783]
EKVX	GI₅₀	1.55 μM Compound: 23, NSC-745885	Growth inhibition of human EKVX cells Growth inhibition of human EKVX cells	[PMID: 19804981]
HCC 2998	GI₅₀	16.4 μM Compound: 23, NSC-745885	Growth inhibition of human HCC2998 cells Growth inhibition of human HCC2998 cells	[PMID: 19804981]
HCT-116	GI₅₀	1.93 μM Compound: 23, NSC-745885	Growth inhibition of human HCT116 cells Growth inhibition of human HCT116 cells	[PMID: 19804981]
HCT-15	GI₅₀	1.71 μM Compound: 23, NSC-745885	Growth inhibition of human HCT15 cells Growth inhibition of human HCT15 cells	[PMID: 19804981]
HL-60(TB)	GI₅₀	0.16 μM Compound: 23, NSC-745885	Growth inhibition of human HL-60(TB) cells Growth inhibition of human HL-60(TB) cells	[PMID: 19804981]
HOP-62	GI₅₀	1.28 μM Compound: 23, NSC-745885	Growth inhibition of human HOP62 cells Growth inhibition of human HOP62 cells	[PMID: 19804981]
HOP-92	GI₅₀	2.52 μM Compound: 23, NSC-745885	Growth inhibition of human HOP92 cells Growth inhibition of human HOP92 cells	[PMID: 19804981]
Hs-578T	GI₅₀	1.41 μM Compound: 23, NSC-745885	Growth inhibition of human Hs 578T cells Growth inhibition of human Hs 578T cells	[PMID: 19804981]
HT-29	GI₅₀	13.4 μM Compound: 23, NSC-745885	Growth inhibition of human HT-29 cells Growth inhibition of human HT-29 cells	[PMID: 19804981]
IGROV-1	GI₅₀	1.36 μM Compound: 23, NSC-745885	Growth inhibition of human IGROV1 cells Growth inhibition of human IGROV1 cells	[PMID: 19804981]
K562	GI₅₀	1.92 μM Compound: 23, NSC-745885	Growth inhibition of human K562 cells Growth inhibition of human K562 cells	[PMID: 19804981]
KM12	GI₅₀	1.96 μM Compound: 23, NSC-745885	Growth inhibition of human KM12 cells Growth inhibition of human KM12 cells	[PMID: 19804981]
LOX IMVI	GI₅₀	0.82 μM Compound: 23, NSC-745885	Growth inhibition of human LOXIMVI cells Growth inhibition of human LOXIMVI cells	[PMID: 19804981]
M14	GI₅₀	2.15 μM Compound: 23, NSC-745885	Growth inhibition of human M14 cells Growth inhibition of human M14 cells	[PMID: 19804981]
Malme-3M	GI₅₀	1.66 μM Compound: 23, NSC-745885	Growth inhibition of human MALME-3M cells Growth inhibition of human MALME-3M cells	[PMID: 19804981]
MCF7	GI₅₀	1.53 μM Compound: 23, NSC-745885	Growth inhibition of human MCF7 cells Growth inhibition of human MCF7 cells	[PMID: 19804981]
MDA-MB-231	GI₅₀	1.75 μM Compound: 23, NSC-745885	Growth inhibition of human MDA-MB-231 cells Growth inhibition of human MDA-MB-231 cells	[PMID: 19804981]
MDA-MB-435	GI₅₀	1.91 μM Compound: 23, NSC-745885	Growth inhibition of human MDA-MB-435 cells Growth inhibition of human MDA-MB-435 cells	[PMID: 19804981]
MOLT-4	GI₅₀	0.49 μM Compound: 23, NSC-745885	Growth inhibition of human MOLT4 cells Growth inhibition of human MOLT4 cells	[PMID: 19804981]
NCI/ADR-RES	GI₅₀	1.86 μM Compound: 23, NSC-745885	Growth inhibition of human NCI/ADR-RES cells Growth inhibition of human NCI/ADR-RES cells	[PMID: 19804981]
NCI-H226	GI₅₀	4.48 μM Compound: 23, NSC-745885	Growth inhibition of human NCI-H226 cells Growth inhibition of human NCI-H226 cells	[PMID: 19804981]
NCI-H23	GI₅₀	1.44 μM Compound: 23, NSC-745885	Growth inhibition of human NCI-H23 cells Growth inhibition of human NCI-H23 cells	[PMID: 19804981]
NCI-H322M	GI₅₀	17.4 μM Compound: 23, NSC-745885	Growth inhibition of human NCI-H322M cells Growth inhibition of human NCI-H322M cells	[PMID: 19804981]
NCI-H460	GI₅₀	3.27 μM Compound: 23, NSC-745885	Growth inhibition of human NCI-H460 cells Growth inhibition of human NCI-H460 cells	[PMID: 19804981]
NCI-H522	GI₅₀	1.66 μM Compound: 23, NSC-745885	Growth inhibition of human NCI-H522 cells Growth inhibition of human NCI-H522 cells	[PMID: 19804981]
OVCAR-3	GI₅₀	0.55 μM Compound: 23, NSC-745885	Growth inhibition of human OVCAR-3 cells Growth inhibition of human OVCAR-3 cells	[PMID: 19804981]
OVCAR-4	GI₅₀	0.65 μM Compound: 23, NSC-745885	Growth inhibition of human OVCAR4 cells Growth inhibition of human OVCAR4 cells	[PMID: 19804981]
OVCAR-5	GI₅₀	1.91 μM Compound: 23, NSC-745885	Growth inhibition of human OVCAR5 cells Growth inhibition of human OVCAR5 cells	[PMID: 19804981]
OVCAR-8	GI₅₀	1.66 μM Compound: 23, NSC-745885	Growth inhibition of human OVCAR8 cells Growth inhibition of human OVCAR8 cells	[PMID: 19804981]
PC-3	GI₅₀	3.23 μM Compound: 23, NSC-745885	Growth inhibition of human PC3 cells Growth inhibition of human PC3 cells	[PMID: 19804981]
PC-3	IC₅₀	2.5 μM Compound: 2, NSC745885	Cytotoxicity against human PC3 cells after 48 hrs by SRB assay Cytotoxicity against human PC3 cells after 48 hrs by SRB assay	[PMID: 26344783]
RXF 393	GI₅₀	1.75 μM Compound: 23, NSC-745885	Growth inhibition of human RXF393 cells Growth inhibition of human RXF393 cells	[PMID: 19804981]
SF-268	GI₅₀	2.17 μM Compound: 23, NSC-745885	Growth inhibition of human SF268 cells Growth inhibition of human SF268 cells	[PMID: 19804981]
SF-295	GI₅₀	1.9 μM Compound: 23, NSC-745885	Growth inhibition of human SF295 cells Growth inhibition of human SF295 cells	[PMID: 19804981]
SF-539	GI₅₀	1.46 μM Compound: 23, NSC-745885	Growth inhibition of human SF539 cells Growth inhibition of human SF539 cells	[PMID: 19804981]
SK-MEL-2	GI₅₀	1.68 μM Compound: 23, NSC-745885	Growth inhibition of human SK-MEL-2 cells Growth inhibition of human SK-MEL-2 cells	[PMID: 19804981]
SK-MEL-28	GI₅₀	1.85 μM Compound: 23, NSC-745885	Growth inhibition of human SK-MEL-28 cells Growth inhibition of human SK-MEL-28 cells	[PMID: 19804981]
SK-MEL-5	GI₅₀	1.58 μM Compound: 23, NSC-745885	Growth inhibition of human SK-MEL-5 cells Growth inhibition of human SK-MEL-5 cells	[PMID: 19804981]
SK-OV-3	GI₅₀	7.71 μM Compound: 23, NSC-745885	Growth inhibition of human SKOV3 cells Growth inhibition of human SKOV3 cells	[PMID: 19804981]
SN12C	GI₅₀	1.33 μM Compound: 23, NSC-745885	Growth inhibition of human SN12C cells Growth inhibition of human SN12C cells	[PMID: 19804981]
SNB-19	GI₅₀	2.28 μM Compound: 23, NSC-745885	Growth inhibition of human SNB19 cells Growth inhibition of human SNB19 cells	[PMID: 19804981]
SNB-75	GI₅₀	2.03 μM Compound: 23, NSC-745885	Growth inhibition of human SNB75 cells Growth inhibition of human SNB75 cells	[PMID: 19804981]
SW-620	GI₅₀	1.79 μM Compound: 23, NSC-745885	Growth inhibition of human SW620 cells Growth inhibition of human SW620 cells	[PMID: 19804981]
T47D	GI₅₀	1.39 μM Compound: 23, NSC-745885	Growth inhibition of human T47D cells Growth inhibition of human T47D cells	[PMID: 19804981]
TK-10	GI₅₀	2.85 μM Compound: 23, NSC-745885	Growth inhibition of human TK10 cells Growth inhibition of human TK10 cells	[PMID: 19804981]
U-251	GI₅₀	1.61 μM Compound: 23, NSC-745885	Growth inhibition of human U251 cells Growth inhibition of human U251 cells	[PMID: 19804981]
UACC-257	GI₅₀	1.74 μM Compound: 23, NSC-745885	Growth inhibition of human UACC257 cells Growth inhibition of human UACC257 cells	[PMID: 19804981]
UACC-62	GI₅₀	1.45 μM Compound: 23, NSC-745885	Growth inhibition of human UACC62 cells Growth inhibition of human UACC62 cells	[PMID: 19804981]
UO-31	GI₅₀	1.62 μM Compound: 23, NSC-745885	Growth inhibition of human UO31 cells Growth inhibition of human UO31 cells	[PMID: 19804981]

In Vitro

NSC745885 (0.5-4 μM; 24, 48 or 72 hours) has a growth inhibitory or death-promoting effect on the SAS cells, it significantly decreases the densities of cultured cells when compared with untreated cells. The IC₅₀ of NSC745885 is 0.85 μM after 72 hours’ treatment^[1].
NSC745885 (0.5-4 μM; 24 hours) increases annexin V positive cells in a dose-dependent manner, and the differences appears as a dose-dependent manner^[1].
NSC745885 (0.5-2 μM; 24 or 48 hours) decreases XIAP protein levels and increases protein levels both as a dose-dependent manner in SAS cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

SAS cells is obtained from a poorly differentiated human squamous cell carcinoma
Concentration:

0.5 μM, 1 μM, 1.5 μM, 2 μM, 4 μM
Incubation Time:

24, 48, or 72 hours
Result:

Decreases SAS cells growth as a time and dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

SAS cells is obtained from a poorly differentiated human squamous cell carcinoma
Concentration:

0.5 μM, 1 μM, 1.5 μM, 2 μM, 4 μM
Incubation Time:

24 hours
Result:

Decreases SAS cells growth as a time and dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

SAS cells is obtained from a poorly differentiated human squamous cell carcinoma
Concentration:

0.5 μM, 1 μM, 1.5 μM, 2 μM
Incubation Time:

24 or 48 hours
Result:

Increased cleaved caspase-3 expression and decreased XIAP expression.

In Vivo

NSC745885 (intraperitoneal injection; 2 mg/kg; once daily; 10 days) treatment significantly reduces tumor size when compared with the vehicle control, and exhibits a higher safety than doxorubicin^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Eight-week-old NOD/SCID (NOD.CB17 Prkdc^scid/J) mice^[1]
Dosage:

2 mg/kg
Administration:

Intraperitoneal injection; 2 mg/kg; once daily; 10 days
Result:

Inhibited engrafted tumors growth in vivo.

Chemical Information

CAS No. 4219-52-7
Appearance Solid
Molecular Weight 266.27
Formula C₁₄H₆N₂O₂S
Color Light yellow to yellow
SMILES

O=C(C1=C(C2=O)C3=NSN=C3C=C1)C4=C2C=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : < 1 mg/mL (insoluble or slightly soluble)

* NSC745885 is usually formulated as a suspension.

View the Complete Stock Solution Preparation Table

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen YW, et al.A novel compound NSC745885 exerts an anti-tumor effect on tongue cancer SAS cells in vitro and in vivo.PLoS One. 2014 Aug 15;9(8):e104703. [Content Brief]

[2]. Tang SH, et al. Pharmacologic down-regulation of EZH2 suppresses bladder cancer in vitro and in vivo.Oncotarget. 2014 Nov 15;5(21):10342-55. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity: