Pleurocidin 

Pleurocidin is an Antimicrobial peptide. Pleurocidin is derived from the skin mucosa or intestinal secretions of Pseudopleuronectes americanus. Pleurocidin inhibits the expression of key proteins in the MAPK and NF-κB inflammatory signaling pathways. Pleurocidin alters serum inflammatory and immune cytokine levels, regulates the down-regulation of tight junction proteins, and modulates the intestinal flora. Pleurocidin exerts antibacterial activity by inducing bacterial membrane damage, hydroxyl radical formation, and NADH depletion, and also produces a synergistic effect with Antibiotics. Pleurocidin alleviates DSS-induced ulcerative colitis. Pleurocidin can be used for research on ulcerative colitis, bacterial infections, and bacterial biofilm-related infections^[1][2][3].

Pleurocidin triggers the expression of upstream genes related to IL-1β and COX-2 in rainbow trout macrophages^[1].
Pleurocidin exhibits antibacterial activity against Escherichia coli in bacterial lytic plate assays^[2].
Pleurocidin (18 h) inhibits the growth of planktonic S. aureus ATCC 25923, E. faecium ATCC 19434, P. acnes ATCC 6919, E. coli ATCC 25922, E. coli O-157 ATCC 43895, and P. aeruginosa ATCC 27853, with an MIC of 1.9-3.8 μg/mL after incubation at 37°C for 18 h^[3].
At its minimum inhibitory concentration (MIC), Pleurocidin (MIC; 30-120 min) transiently increases the NAD⁺/NADH ratio of planktonic S. aureus ATCC 25923 and P. aeruginosa ATCC 27853 to more than 4 times the original level at 30 min post-treatment, and this ratio returns to the baseline level at 60 min^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pleurocidin (5 mg/kg; rectal injection; 4 doses) significantly alleviates DSS-induced ulcerative colitis in BALB/C6J mice by reducing clinical symptoms, improving colonic histopathology, regulating inflammatory cytokines and signaling pathways, enhancing intestinal barrier function, and modulating gut microbiota composition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2711.13

C₁₂₉H₁₉₂N₃₆O₂₉

190324-47-1

Solid

White to off-white

Gly-Trp-Gly-Ser-Phe-Phe-Lys-Lys-Ala-Ala-His-Val-Gly-Lys-His-Val-Gly-Lys-Ala-Ala-Leu-Thr-His-Tyr-Leu

GWGSFFKKAAHVGKHVGKAALTHYL

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Nong K, et al. Effect of the Pseudopleuronectes americanus-derived Pleurocidin on DSS-induced Ulcerative colitis in mice and its preliminary molecular mechanisms. Int Immunopharmacol. 2024;130:111757.

  [2]. Cole AM, et al. Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. J Biol Chem. 1997;272(18):12008-12013.  [Content Brief]

  [3]. Choi H, et al. Antimicrobial peptide pleurocidin synergizes with antibiotics through hydroxyl radical formation and membrane damage, and exerts antibiofilm activity. Biochim Biophys Acta. 2012;1820(12):1831-1838.