Proteasome-activating peptide 1 TFA

Name: Proteasome-activating peptide 1 TFA
Brand: MedChemExpress
SKU: HY-P3414A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P3414A Purity: 99.82%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Proteasome-activating peptide 1 TFA is a peptide and a potent proteasome activator. Proteasome-activating peptide 1 TFA increases the chymotrypsin-like proteasomal catalytic activity and, consequently, proteolytic rates both in vitro and in culture. Proteasome-activating peptide 1 TFA prevents protein aggregation in a cellular model of amyotrophic lateral sclerosis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Proteasome-activating peptide 1 TFA Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 470	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Proteasome-activating peptide 1 TFA

•Cell Signal. 2023 Dec 17:114:111015. [Abstract]

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Proteasome-activating peptide 1 (TFA) (0-100 μM, 2 h) increases ChT-L catalytic activity of the 20S proteasome in a dose-dependent manner^[1].
Proteasome-activating peptide 1 (TFA) (50 μM, 24 h) induces the degradation of SOD1, promotes a decrease of oxidized proteins^[1].
Proteasome-activating peptide 1 (TFA) (0-100 μM) protects fibroblast and SH-SY5Y cells from oxidative stress^[1].
Proteasome-activating peptide 1 (TFA) only affects the free 20S proteasomal pool^[1].
Proteasome-activating peptide 1 (TFA) promotes proteasomal gate opening^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Human neuroblastoma (SH-SY5Y) and fibroblast cells^[1]
Concentration:	0, 50, 100 μM (in DMEM without fetal bovine serum followed by the addition of H2O2 (500 or 750 μM) or water (0.4%))
Incubation Time:	30 min or 2 h
Result:	Promoted long-term proteasomal activation through interaction with the 20S core particle, increased ChT-L activity of the 20S proteasome in a dose-dependent manner, and did not reduce cell fibroblast viability.

Western Blot Analysis

Cell Line:	SH-SY5Y^G93A cells^[1]
Concentration:	50 μM
Incubation Time:	24 h
Result:	Increased the degradation of soluble SOD1, prevented the formation of SOD1-insoluble aggregates, and decreased the oxidized proteins in the total intracellular content.

Molecular Weight

1632.02

Formula

C₆₅H₁₁₇F₃N₂₂O₁₅S₄

Appearance

Solid

Color

White to off-white

Sequence Shortening

IPRCRKMPGVKMC-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (61.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (30.64 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.6127 mL	3.0637 mL	6.1274 mL
5 mM	0.1225 mL	0.6127 mL	1.2255 mL
10 mM	0.0613 mL	0.3064 mL	0.6127 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.82%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (241 KB) LCMS (229 KB) Elemental Analysis Report (102 KB)

Handling Instructions (2659 KB)

References

[1]. Dal Vechio FH, et al. Peptides that activate the 20S proteasome by gate opening increased oxidized protein removal and reduced protein aggregation. Free Radic Biol Med. 2014;67:304-313. [Content Brief]

[2]. Dal Vechio FH, Cerqueira F, Augusto O, Lopes R, Demasi M. Peptides that activate the 20S proteasome by gate opening increased oxidized protein removal and reduced protein aggregation. Free Radic Biol Med. 2014 Feb;67:304-13. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.6127 mL	3.0637 mL	6.1274 mL	15.3184 mL
	5 mM	0.1225 mL	0.6127 mL	1.2255 mL	3.0637 mL
	10 mM	0.0613 mL	0.3064 mL	0.6127 mL	1.5318 mL
	15 mM	0.0408 mL	0.2042 mL	0.4085 mL	1.0212 mL
	20 mM	0.0306 mL	0.1532 mL	0.3064 mL	0.7659 mL
	25 mM	0.0245 mL	0.1225 mL	0.2451 mL	0.6127 mL
	30 mM	0.0204 mL	0.1021 mL	0.2042 mL	0.5106 mL
DMSO	40 mM	0.0153 mL	0.0766 mL	0.1532 mL	0.3830 mL
	50 mM	0.0123 mL	0.0613 mL	0.1225 mL	0.3064 mL
	60 mM	0.0102 mL	0.0511 mL	0.1021 mL	0.2553 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.