Antibacterial and cytotoxic triterpenoids from the roots of Combretum racemosum

  • Fitoterapia. 2016 Apr;110:89-95. doi: 10.1016/j.fitote.2016.03.002.
Diane Patricia Apie Gossan  1 ,  Abdulmagid Alabdul Magid  2 ,  Philomène Akoua Yao-Kouassi  3 ,  Jérôme Josse  4 ,  Sophie C Gangloff  4 ,  Hamid Morjani  5 ,  Laurence Voutquenne-Nazabadioko  6
Affiliations
  • 1. ICMR-UMR CNRS 7312, Groupe Isolement et Structure, Campus Sciences, Bât. 18, BP 1039, 51687 Reims Cedex 2, France; Laboratoire de Chimie Organique et de Substances Naturelles, 22 BP 582 Abidjan 22, Université Félix-Houphouët Boigny d'Abidjan-Cocody, Ivory Coast, France.
  • 2. ICMR-UMR CNRS 7312, Groupe Isolement et Structure, Campus Sciences, Bât. 18, BP 1039, 51687 Reims Cedex 2, France. Electronic address: [email protected].
  • 3. Laboratoire de Chimie Organique et de Substances Naturelles, 22 BP 582 Abidjan 22, Université Félix-Houphouët Boigny d'Abidjan-Cocody, Ivory Coast, France.
  • 4. Laboratoire de microbiologie, EA 4691, « Biomatériaux et inflammation en site osseux », UFR de pharmacie, 1, rue du Maréchal-Juin, 51096 Reims Cedex, France.
  • 5. MEDyC UMR CNRS 7369, URCA, Faculté de Pharmacie, SFR CAP Santé, Reims, France.
  • 6. ICMR-UMR CNRS 7312, Groupe Isolement et Structure, Campus Sciences, Bât. 18, BP 1039, 51687 Reims Cedex 2, France.
PMID: 26946378 DOI: 10.1016/j.fitote.2016.03.002
Abstract

A new pentacyclic triterpenoid glucoside, together with fourteen known compounds, was isolated from the roots of Combretum racemosum. Combretaceae). The structure of the new compound was established as 28-O-β-d-glucopyranosyl-2α,3β,21β,23-tetrahydroxyolean-18-en-28-oate (1) on the basis of detailed spectroscopic data including MS, 1D, and 2D NMR. The inhibitory activity of compounds 1-15 against promyelocytic leukemia HL-60 and human erythromyeloblastoid leukemia K562 cell lines was evaluated. Compounds 11 (3-O-β-acetyl-ursolic acid), 14 (betulinic acid), and 15 (quadranoside II) exhibited significant cytotoxicity, with IC50 values of 13 to 50 μM. Among the isolated Triterpenes, compounds 1, 3 (arjungenin), 5 (terminolic acid), and 11 exhibited moderate Antibacterial activity against Staphylococcus aureus, Escherichia coli and Enterococcus faecalis (MICs within a range of 64 and 256 μg/mL).

Keywords
Antibacterial activity; Combretaceae; Combretum racemosum; Cytotoxic activity; Triterpenes.
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