Asperflavin, an Anti-Inflammatory Compound Produced by a Marine-Derived Fungus, Eurotium amstelodami

  • Molecules. 2017 Oct 29;22(11):1823. doi: 10.3390/molecules22111823.
Xiudong Yang  1  2 ,  Min-Cheol Kang  3 ,  Yong Li  4 ,  Eun-A Kim  5 ,  Sung-Myung Kang  6 ,  You-Jin Jeon  7  8
Affiliations
  • 1. College of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China. [email protected].
  • 2. Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. [email protected].
  • 3. Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. [email protected].
  • 4. College of Pharmacy Science, Changchun University of Traditional Chinese Medicine, Changchun 130017, China. [email protected].
  • 5. Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. [email protected].
  • 6. Pediatric Oncology Experimental Therapeutics Investigators Consortium (POETIC) Laboratory for Pre-Clinical and Drug Discovery Studies, University of Calgary, Calgary, AB T2N1N4, Canada. [email protected].
  • 7. Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea. [email protected].
  • 8. Marine and Environmental Research Institute, Jeju National University, Jeju 695-814, Korea. [email protected].
PMID: 29109367 DOI: 10.3390/molecules22111823
Abstract

In the present study, 16 marine-derived fungi were isolated from four types of marine Materials including float, algae, Animals and drift woods along with the coast of Jeju Island, Korea and evaluated for anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW 24.7 cells. The broth and mycelium extracts from the 16 fungi were prepared and the broth extract (BE) of Eurotium amstelodami (015-2) inhibited nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells without cytotoxicity. By further bioassay-guided isolation, three compounds including asperflavin, neoechinulin A and preechinulin were successfully isolated from the BE of E. amstelodami. It was revealed that asperflavin showed no cytotoxicity up to 200 μM and significantly inhibited LPS-induced NO and PGE2 production in a dose-dependent manner. In the western blot results, asperflavin suppressed only inducible NOS (iNOS), but COX-2 were slightly down-regulated. Asperflavin was also observed to inhibit the production of pro-inflammatory cytokines including TNF-α, IL-1β, and IL-6. In conclusion, this study reports a potential use of asperflavin isolated from a marine fungus, E. amstelodami as an anti-inflammatory agent via suppression of iNOS and pro-inflammatory cytokines as well as no cytotoxicity.

Keywords
Anti-inflammation; Eurotium amstelodami; NO production; asperflavin; marine fungus.
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