ABHD10 Protein, Human (sf9, His)
The ABHD10 protein is an acylprotein thioesterase that hydrolyzes fatty acids from acylated residues in proteins. It regulates mitochondrial S-depalmitoylation of peroxiredoxin-5/PRDX5 and modulates mitochondrial antioxidant capacity. ABHD10 Protein, Human (sf9, His) is the recombinant human-derived ABHD10 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ABHD10 protein is an acylprotein thioesterase that hydrolyzes fatty acids from acylated residues in proteins. It regulates mitochondrial S-depalmitoylation of peroxiredoxin-5/PRDX5 and modulates mitochondrial antioxidant capacity. ABHD10 Protein, Human (sf9, His) is the recombinant human-derived ABHD10 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
ABHD10 protein serves as an acyl-protein thioesterase, playing a crucial role in hydrolyzing fatty acids from acylated residues in proteins. Notably, it regulates the mitochondrial S-depalmitoylation of the nucleophilic active site residue of peroxiredoxin-5/PRDX5, a key antioxidant protein, thereby modulating the antioxidant capacity of mitochondria. Beyond its involvement in redox homeostasis, ABHD10 catalyzes the deglucuronidation of mycophenolic acid acyl-glucuronide, which is an active metabolite of the immunosuppressant drug mycophenolate. This dual functionality underscores the diverse regulatory impact of ABHD10 in cellular processes related to lipid metabolism, mitochondrial function, and drug metabolism.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
Q9NUJ1-1 (T53-N306)
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Molecular Construction
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N-term
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His
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ABHD10 (T53-N306)
Accession # Q9NUJ1-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
ABHD10; Alpha/Beta Hydrolase Domain-Containing Protein 10, Mitochondrial; Abhydrolase Domain Containing 10, Depalmitoylase; Abhydrolase Domain-Containing Protein 10, Mitochondrial; Mycophenolic Acid Acyl-Glucuronide Esterase, Mitochondrial; Alpha/Beta Hyd
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AA Sequence
TSLSFLNRPDLPNLAYKKLKGKSPGIIFIPGYLSYMNGTKALAIEEFCKSLGHACIRFDYSGVGSSDGNSEESTLGKWRKDVLSIIDDLADGPQILVGSSLGGWLMLHAAIARPEKVVALIGVATAADTLVTKFNQLPVELKKEVEMKGVWSMPSKYSEEGVYNVQYSFIKEAEHHCLLHSPIPVNCPIRLLHGMKDDIVPWHTSMQVADRVLSTDVDVILRKHSDHRMREKADIQLLVYTIDDLIDKLSTIVN
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Predicted Molecular Mass
30.3 kDa
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Molecular Weight
Approximately 32 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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