ACAT1 Protein, Human
The ACAT1 protein is a key enzyme in mitochondrial beta-oxidation and plays a crucial role in breaking down fatty acids into acetyl-CoA. In the final step, ACAT1 catalyzes the thiolytic cleavage of 3-oxoacyl-CoA to generate acetyl-CoA and shortened fatty acyl-CoA. ACAT1 Protein, Human is the recombinant human-derived ACAT1, expressed by E. coli, with tag-free. The total length of ACAT1 Protein, Human is 394 a.a..
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The ACAT1 protein is a key enzyme in mitochondrial beta-oxidation and plays a crucial role in breaking down fatty acids into acetyl-CoA. In the final step, ACAT1 catalyzes the thiolytic cleavage of 3-oxoacyl-CoA to generate acetyl-CoA and shortened fatty acyl-CoA. ACAT1 Protein, Human is the recombinant human-derived ACAT1, expressed by E. coli, with tag-free. The total length of ACAT1 Protein, Human is 394 a.a..
Background
The ACAT1 protein is a pivotal enzyme in the mitochondrial beta-oxidation pathway, a vital aerobic process responsible for breaking down fatty acids into acetyl-CoA. Operating in the last step of this pathway, ACAT1 utilizes free coenzyme A (CoA) to catalyze the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs, generating acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. This reversible enzymatic activity extends to the condensation of two acetyl-CoA molecules into acetoacetyl-CoA, underscoring ACAT1's essential role in ketone body metabolism. The multifaceted functions of ACAT1 illuminate its significance in regulating key metabolic processes, shedding light on its integral role in maintaining cellular energy balance and homeostasis.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P24752-1 (V34-L427)
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Gene ID38
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Protein Length
Full Length of Isoform-1
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Synonyms
ACAT1; Acetyl-Coenzyme A Acetyltransferase 1; Prev. ACAT; MAT; Acetyl-CoA Acetyltransferase, Mitochondrial; T2; Acetoacetyl-CoA Thiolase; Mitochondrial Acetoacetyl-CoA Thiolase; THIL; Testicular Tissue Protein Li 198; Acetyl-CoA Acetyltransferase 1; Aceto
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AA Sequence
VSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIARNEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLEIDPQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQKL
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Predicted Molecular Mass
41.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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