ACAT2 Protein, Rat (His)
Based on 1 Customer Validation
ACAT2 Protein is pivotal in cholesterol biosynthesis, intricately contributing to cellular lipid homeostasis regulation. Its involvement underscores its significance in modulating cholesterol levels, highlighting an essential function in cellular physiology. ACAT2 Protein, Rat (His) is the recombinant rat-derived ACAT2 protein, expressed by E. coli , with N-His labeled tag.
- Species: Rat
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
ACAT2 Protein is pivotal in cholesterol biosynthesis, intricately contributing to cellular lipid homeostasis regulation. Its involvement underscores its significance in modulating cholesterol levels, highlighting an essential function in cellular physiology. ACAT2 Protein, Rat (His) is the recombinant rat-derived ACAT2 protein, expressed by E. coli , with N-His labeled tag.
The ACAT2 Protein plays a pivotal role in the biosynthetic pathway of cholesterol. As an integral component of this metabolic process, ACAT2 is involved in the intricate synthesis of cholesterol, contributing to the regulation and maintenance of cellular lipid homeostasis. The enzyme's involvement highlights its significance in modulating cholesterol levels and underscores its essential function in cellular physiology.
Measured by its ability to convert acetoacetyl-CoA and CoA into acetyl-CoA. The specific activity is 7519.724 pmol/min/µg that incubate at room temperature in kinetic mode for 10 minutes.
Assay Procedure
Materials
Assay buffer: 50 mM Tris, 25 mM MgCl2, 50 mM KCl, 0.5 mM DTT, pH 8.0
ACAT2 Protein, Rat (His) (HY-P75543) ACAT2
Coenzyme A Sodium Salt Hydrate (CoA)
Acetoacetyl Coenzyme A Sodium Salt Hydrate (AACoA)
Procedure
1. Dilute Rat ACAT2 to 0.05 μg/mL in assay buffer.
2. Prepare substrate mixture containing 100 μM CoA and 30 μM AACoA in assay buffer.
3. Experimental group: 50 μL protein + 50 μL substrate.
Blank group: 50 μL assay buffer + 50 μL substrate.
4. Read in kinetic mode for 10 minutes at an absorbance of 303 nm.
5. Calculate specific activity:
Specific Activity (pmol/min/μg) = | Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol |
| ext. coeff ** (M-1cm-1) x path corr. *** (cm) x amount of enzyme (μg) |
*Adjusted for substrate blank
**Extinction coefficient: 16900 M-1cm-1
***Path correction: 0.32 cm
Per Well:
Rat ACAT2: 0.0025 μg
CoA: 50 μM
AACoA: 15 μM
Technical Parameters
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Species Rat
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Source E. coli
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Tag N-6*His
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Accession
Q5XI22 (M1-G397)
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Molecular Construction
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N-term
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His
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ACAT2 (M1-G397)
Accession # Q5XI22 -
C-term
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Protein Length
Full Length
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Synonyms
ACAT2; Acetoacetyl Coenzyme A Thiolase; Acetyl-CoA Acetyltransferase 2; Acetyl-Coenzyme A Acetyltransferase 2 (Acetoacetyl Coenzyme A Thiolase); Acetyl-CoA Acetyltransferase, Cytosolic; Acetyl-Coenzyme A Acetyltransferase 2; Acetyl-CoA Transferase-Like Pr
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AA Sequence
MNAGSDPVVIISAARTAIGSFNGALSTVPVHNLGTTVIKEVLQRAKVAPEEVSEVIFGHVLTAGCGQNPTRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAAQSIAMGDSTIVVAGGMENMSKAPHLAHLRSGVKMGEVPLADSILCDGLTDAFHNYHMGITAENVAKKWQVSREAQDKVAVVSQNRAEHAQKAGHFDKEIVPVHVSSRKGLTEVKIDEFPRHGSNLEAMSKLKPYFLTDGTGTVTPANASGMNDGAAAVVLMKKTEAESRMLKPLAQVVSWSQAGVEPSVMGVGPIPAIKQAVAKAGWSLEDVDVFEINEAFAAVSAAIAKELGLSPEKVNIDGGAIALGHPLGASGCRILVTLLHTLERVGGTRGVAALCIGGGMGIAMCVQRG
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Molecular Weight
Approximately 43.3 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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