Copy Link Print Information

ACOX1 Protein, Human (sf9, His)

Customer Review

Based on 1 Customer Validation

The ACOX1 protein is critical in the initial steps of peroxisomal β-oxidation, especially of very long-chain fatty acids. It catalyzes the desaturation of fatty acyl-CoA (such as palmitoyl-CoA) to 2-trans-enoyl-CoA. ACOX1 Protein, Human (sf9, His) is the recombinant human-derived ACOX1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for ACOX1 Protein, Human (sf9, His)
  • ACOX1 Protein, Human (sf9, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The ACOX1 protein is critical in the initial steps of peroxisomal β-oxidation, especially of very long-chain fatty acids. It catalyzes the desaturation of fatty acyl-CoA (such as palmitoyl-CoA) to 2-trans-enoyl-CoA. ACOX1 Protein, Human (sf9, His) is the recombinant human-derived ACOX1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

The ACOX1 Protein plays a pivotal role in the initial and rate-limiting step of peroxisomal beta-oxidation, particularly of straight-chain saturated and unsaturated very-long-chain fatty acids. Its catalytic activity involves the desaturation of fatty acyl-CoAs, such as palmitoyl-CoA, into 2-trans-enoyl-CoAs, exemplified by (2E)-hexadecenoyl-CoA. Additionally, ACOX1 contributes to the production of hydrogen peroxide (H(2)O(2)) by donating electrons directly to molecular oxygen (O(2)). While displaying notable activity against medium-chain fatty acyl-CoAs, its optimum activity is observed with a chain length of 10 carbons, exemplified by decanoyl-CoA, as demonstrated in vitro. This multifaceted enzymatic activity underscores ACOX1's integral role in peroxisomal beta-oxidation and the metabolism of various fatty acid substrates.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-10*His
  • Accession

    AAH08767.1 (M1-L660)

  • Gene ID
    51  [NCBI]
  • Molecular Construction
    • N-term
    • 10*His
    • ACOX1 (M1-L660)
      Accession # Q15067
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    ACOX1; Acyl-CoA Oxidase 1, Palmitoyl; Acyl-CoA Oxidase 1; SCOX; Palmitoyl-CoA Oxidase; ACOX; Peroxisomal Acyl-Coenzyme A Oxidase 1; AOX; Peroxisomal Fatty Acyl-CoA Oxidase; Acyl-CoA Oxidase, Straight-Chain; Straight-Chain Acyl-CoA Oxidase; Acyl-Coenzyme A Oxidase; PALMCOX; MITCH; Acyl-Coenzyme A Oxidase 1, Palmitoyl

  • AA Sequence

    MNPDLRRERDSASFNPELLTHILDGSPEKTRRRREIENMILNDPDFQHEDLNFLTRSQRYEVAVRKSAIMVKKMREFGIADPDEIMWFKNFVHRGRPEPLDLHLGMFLPTLLHQATAEQQERFFMPAWNLEIIGTYAQTEMGHGTHLRGLETTATYDPETQEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITKGKCYGLHAFIVPIREIGTHKPLPGITVGDIGPKFGYDEIDNGYLKMDNHRIPRENMLMKYAQVKPDGTYVKPLSNKLTYGTMVFVRSFLVGEAARALSKACTIAIRYSAVRHQSEMKPGEPEPQILDFQTQQYKLFPLLATAYAFQFVGAYMKETYHRINEGIGQGDLSELPELHALTAGLKAFTSWTANTGIEACRMACGGHGYSHCSGLPNIYVNFTPSCTFEGENTVMMLQTARFLMKSYDQVHSGKLVCGMVSYLNDLPSQRIQPQQVAVWPTMVDINSPESLTEAYKLRAARLVEIAAKNLQKEVIHRKSKEVAWNLTSVDLVRASEAHCHYVVVKLFSEKLLKIQDKAIQAVLRSLCLLYSLYGISQNAGDFLQGSIMTEPQITQVNQRVKELLTLIRSDAVALVDAFDFQDVTLGSVLGRYDGNVYENLFEWAKNSPLNKAEVHESYKHLKSLQSKL

  • Predicted Molecular Mass

    76.7 kDa

  • Molecular Weight

    Approximately 75 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.0, 20% glycerol, 3 mM DTT.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, 0.5 mM TCEP, pH 7.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • ACOX1 Protein, Human (sf9, His) Product Image
    1/16
  • Experimental Validation Results for ACOX1 Protein, Human (sf9, His)
  • ACOX1 Protein, Human (sf9, His) Product Image