ACSL1 Protein, Human (His)
Based on 1 Customer Validation
ACSL1 Protein, Human (His) is the recombinant human-derived ACSL1 protein, expressed by E. coli, with C-6*His tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
ACSL1 Protein, Human (His) is the recombinant human-derived ACSL1 protein, expressed by E. coli, with C-6*His tag.
ACSL1 catalyzes the conversion of long-chain fatty acids into their active form acyl-CoAs, which are utilized for cellular lipid synthesis and degradation via beta-oxidation. It shows a preference for palmitoleate, oleate, and linoleate. Additionally, it preferentially activates arachidonate over epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (by similar).
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P33121 (T46-V698)
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Gene ID2180
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Molecular Construction
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N-term
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ACSL1 (T46-V698)
Accession # P33121 -
6*His
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C-term
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Protein Length
Cytoplasmic Domain
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Synonyms
ACSL1; Lignoceroyl-CoA Synthase; Prev. FACL2; Palmitoyl-CoA Ligase 1; LACS2; Palmitoyl-CoA Ligase 2; LACS1; Acyl-CoA Synthetase 1; FACL1; Phytanate--CoA Ligase; LACS; LACS 1; ACS1; LACS 2; Fatty-Acid-Coenzyme A Ligase, Long-Chain 2; Fatty-Acid-Coenzyme A
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AA Sequence
TRPKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQRGIQVSNNGPCLGSRKPDQPYEWLSYKQVAELSECIGSALIQKGFKTAPDQFIGIFAQNRPEWVIIEQGCFAYSMVIVPLYDTLGNEAITYIVNKAELSLVFVDKPEKAKLLLEGVENKLIPGLKIIVVMDAYGSELVERGQRCGVEVTSMKAMEDLGRANRRKPKPPAPEDLAVICFTSGTTGNPKGAMVTHRNIVSDCSAFVKATENTVNPCPDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTVFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDRLIFHKVQSSLGGRVRLMVTGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTMPGDWTAGHVGAPMPCNLIKLVDVEEMNYMAAEGEGEVCVKGPNVFQGYLKDPAKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYMRSEPVAQVFVHGESLQAFLIAIVVPDVETLCSWAQKRGFEGSFEELCRNKDVKKAILEDMVRLGKDSGLKPFEQVKGITLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDDLYSTIKV
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Predicted Molecular Mass
79.5 kDa
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Molecular Weight
Approximately 80 kDa, based on SDS-PAGE under reducing conditions.
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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