ADAM15 Protein, Mouse (HEK293, His)

ADAM15 is an active metalloprotease that plays a crucial role in physiological processes and has gelatin-solubilizing and collagen-solubilizing activities. Notably, it affects wound healing, mediates intraepithelial cell/T cell interactions, and inhibits β-1 integrin-mediated cell adhesion and migration in airway smooth muscle cells. ADAM15 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM15 protein, expressed by HEK293 , with C-His labeled tag. The total length of ADAM15 Protein, Mouse (HEK293, His) is 680 a.a., with molecular weight of 75-110 kDa.

ADAM15, an active metalloproteinase, exhibits gelatinolytic and collagenolytic activity, playing a crucial role in diverse physiological processes. Its involvement in wound healing is notable, and it mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Additionally, ADAM15 functions in inhibiting beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells, suppressing cell motility on or towards fibronectin by potentially influencing alpha-v/beta-1 integrin cell surface expression through ERK1/2 inactivation. Notably, ADAM15 cleaves E-cadherin in response to growth factor deprivation and contributes to glomerular cell migration, suggesting its involvement in various cellular events. Furthermore, its role in pathological neovascularization and potential contribution to cartilage remodeling underscore its multifaceted functions. ADAM15 may undergo proteolytic processing during sperm epididymal maturation and the acrosome reaction, potentially playing a role in sperm-egg binding through interactions with egg membrane receptors, particularly integrins.

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 4.53 μg/mL, corresponding to a specific activity is 2.21×10² units/mg.

Mouse

HEK293

C-10*His

O88839 (R18-T697)

11490  [NCBI]

RPSPPLPNIGGTEEEQQASPERTLSGSMESRVVQDSPPMSLADVLQTGLPEALRISLELDSESHVLELLQNRDLIPGRPTLVWYQPDGTRMVSEGYSLENCCYRGRVQGHPSSWVSLCACSGIRGLIVLSPERGYTLELGPGDLQRPVISRIQDHLLLGHTCAPSWHASVPTRAGPDLLLEQHHAHRLKRDVVTETKIVELVIVADNSEVRKYPDFQQLLNRTLEAALLLDTFFQPLNVRVALVGLEAWTQHNLIEMSSNPAVLLDNFLRWRRTDLLPRLPHDSAQLVTVTSFSGPMVGMAIQNSICSPDFSGGVNMDHSTSILGVASSIAHELGHSLGLDHDSPGHSCPCPGPAPAKSCIMEASTDFLPGLNFSNCSRQALEKALLEGMGSCLFERQPSLAPMSSLCGNMFVDPGEQCDCGFPDECTDPCCDHFTCQLRPGAQCASDGPCCQNCKLHPAGWLCRPPTDDCDLPEFCPGDSSQCPSDIRLGDGEPCASGEAVCMHGRCASYARQCQSLWGPGAQPAAPLCLQTANTRGNAFGSCGRSPGGSYMPCAPRDVMCGQLQCQWGRSQPLLGSVQDRLSEVLEANGTQLNCSWVDLDLGNDVAQPLLALPGTACGPGLVCIGHRCQPVDLLGAQECRRKCHGHGVCDSSGHCRCEEGWAPPDCMTQLKATSSLTT

Approximately 75-110 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.