ADAM8 Protein, Human (HEK293, His)
Based on 1 Customer Validation
ADAM8 protein is suggested to be involved in leukocyte extravasation, indicating its potential role in facilitating the migration of white blood cells from blood vessels into surrounding tissues. This implies ADAM8's contribution to immune responses by regulating leukocyte movement across the endothelial barrier, a crucial step in inflammatory and immune surveillance processes. ADAM8 Protein, Human (HEK293, His) is the recombinant human-derived ADAM8 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ADAM8 protein is suggested to be involved in leukocyte extravasation, indicating its potential role in facilitating the migration of white blood cells from blood vessels into surrounding tissues. This implies ADAM8's contribution to immune responses by regulating leukocyte movement across the endothelial barrier, a crucial step in inflammatory and immune surveillance processes. ADAM8 Protein, Human (HEK293, His) is the recombinant human-derived ADAM8 protein, expressed by HEK293 , with C-His labeled tag.
Background
ADAM8 protein is speculated to participate in the process of leukocyte extravasation, implying its potential role in facilitating the migration of white blood cells from blood vessels into surrounding tissues. This suggests that ADAM8 may contribute to immune responses by regulating the movement of leukocytes across the endothelial barrier, an essential step in the inflammatory and immune surveillance processes.
Verified Bioactivity
1.Immobilized Human ADAM8, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of ≤8.1 ng/mL determined by ELISA.
2.Immobilized Human ADAM8, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 1.0-10.0 ng/mL determined by ELISA.
3.Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSRNH2. The specific activity is >5 pmol/min/µg.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-His
-
Accession
AAI15405.1 (I17-P655)
-
Molecular Construction
-
N-term
-
ADAM8 (I17-P655)
Accession # P78325 -
His
-
C-term
-
-
Protein Length
Partial
-
Synonyms
ADAM8; Cell Surface Antigen MS2; ADAM Metallopeptidase Domain 8; CDNA, FLJ93272, Highly Similar To Homo Sapiens A Disintegrin And Metalloproteinase Domain 8 (ADAM8),MRNA; MS2; Human Leukocyte Differentiation Antigen; CD156A; CD156a Antigen; CD156; ADAM8 P
-
AA Sequence
IAPSRPWALMEQYEVVLPRRLPGPRVRRALPSHLGLHPERVSYVLGATGHNFTLHLRKNRDLLGSGYTETYTAANGSEVTEQPRGQDHCFYQGHVEGYPDSAASLSTCAGLRGFFQVGSDLHLIEPLDEGGEGGRHAVYQAEHLLQTAGTCGVSDDSLGSLLGPRTAAVFRPRPGDSLPSRETRYVELYVVVDNAEFQMLGSEAAVRHRVLEVVNHVDKLYQKLNFRVVLVGLEIWNSQDRFHVSPDPSVTLENLLTWQARQRTRRHLHDNVQLITGVDFTGTTVGFARVSAMCSHSSGAVNQDHSKNPVGVACTMAHEMGHNLGMDHDENVQGCRCQERFEAGRCIMAGSIGSSFPRMFSDCSQAYLESFLERPQSVCLANAPDLSHLVGGPVCGNLFVERGEQCDCGPPEDCRNRCCNSTTCQLAEGAQCAHGTCCQECKVKPAGELCRPKKDMCDLEEFCDGRHPECPEDAFQENGTPCSGGYCYNGACPTLAQQCQAFWGPGGQAAEESCFSYDILPGCKASRYRADMCGVLQCKGGQQPLGRAICIVDVCHALTTEDGTAYEPVPEGTRCGPEKVCWKGRCQDLHVYRSSNCSAQCHNHGVCNHKQECHCHAGWAPPHCAKLLTEVHAASGSLP
-
Predicted Molecular Mass
70.9 kDa
-
Molecular Weight
Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (240 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
