ADRA2A-VLPs Protein, Human (HEK293, His)
Based on 1 Customer Validation
ADRA2A-VLPs Protein, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ADRA2A-VLPs Protein, Human (HEK293, His) is recommended for animal immunization, ELISA. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.
Background
ADRA2A-VLPs Protein, representing alpha-2 adrenergic receptors, play a crucial role in mediating the inhibition of adenylate cyclase induced by catecholamines through G protein action. The potency order for agonists targeting this receptor includes oxymetazoline, clonidine, epinephrine, norepinephrine, phenylephrine, dopamine, p-synephrine, p-tyramine, serotonin, and p-octopamine. Conversely, the rank order for antagonists comprises yohimbine, phentolamine, mianserine, chlorpromazine, spiperone, prazosin, propranolol, alprenolol, and pindolol. These findings delineate the pharmacological profile of ADRA2A-VLPs, shedding light on their responsiveness to various agonists and antagonists and providing insights into the intricate regulatory mechanisms governing adenylate cyclase inhibition in response to catecholamine signaling.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
P08913 (M1-V465)
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Gene ID150
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Molecular Construction
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N-term
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ADRA2A-VLPs (M1-V465)
Accession # P08913 -
10*His
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C-term
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Protein Length
Full Length
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Synonyms
ADRA2A; Alpha-2A Adrenoreceptor; Prev. ADRA2R; Alpha-2A Adrenoceptor; Prev. ADRA2; Alpha-2-Adrenergic Receptor, Platelet Type; ADRAR; Alpha-2A-Adrenergic Receptor; Alpha-2 Adrenergic Receptor Subtype C10; Alpha-2AAR; Adrenergic, Alpha-2A-, Receptor; ALPHA
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AA Sequence
MFRQEQPLAEGSFAPMGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITVWVISAVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGPGATGIGTPAAGPGEERVGAAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVGCSVPRTLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV
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Predicted Molecular Mass
52 kDa
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Glycosylation
Yes
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Purity
Purity analysis by SDS-PAGE is not available for VLP proteins.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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