ALDH3A1 Protein, Human (HEK293, His)

ALDH3A1 Protein, Human (HEK293, His) is a human recombinant ALDH3A1 with a His tag at the C-terminus. ALDH3A1 Protein, Human (HEK293, His) is expressed in HEK293 cells.

Aldehyde dehydrogenase 3A1 (ALDH3A1) is an important member of the ALDH superfamily, which consists of NAD(P)+- dependent enzymes that oxidize a wide variety of endogenously produced and exogenously derived aldehydes to their corresponding carboxylic acids. ALDH3A1 is often overexpressed in esophagus-mucosa, minor salivary gland, nasal epithelium, tonsil and oral epithelium. ALDH3A1 has been shown to be upregulated in several cancer types, such as stomach cancer, lung cancer or oral squamous cell carcinoma (OSCC)^[1].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P30838 (M1-H453)

218  [NCBI]

Full Length

MSKISEAVKRARAAFSSGRTRPLQFRIQQLEALQRLIQEQEQELVGALAADLHKNEWNAYYEEVVYVLEEIEYMIQKLPEWAADEPVEKTPQTQQDELYIHSEPLGVVLVIGTWNYPFNLTIQPMVGAIAAGNSVVLKPSELSENMASLLATIIPQYLDKDLYPVINGGVPETTELLKERFDHILYTGSTGVGKIIMTAAAKHLTPVTLELGGKSPCYVDKNCDLDVACRRIAWGKFMNSGQTCVAPDYILCDPSIQNQIVEKLKKSLKEFYGEDAKKSRDYGRIISARHFQRVMGLIEGQKVAYGGTGDAATRYIAPTILTDVDPQSPVMQEEIFGPVLPIVCVRSLEEAIQFINQREKPLALYMFSSNDKVIKKMIAETSSGGVAANDVIVHITLHSLPFGGVGNSGMGSYHGKKSFETFSHRRSCLVRPLMNDEGLKVRYPPSPAKMTQH

Approximately 54.0 kDa

≥ 80%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 15% trehalose, 4% Mannitol, 50 mM NaCl, 0.1% Tween80, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Qu Y, et al. ALDH3A1 acts as a prognostic biomarker and inhibits the epithelial mesenchymal transition of oral squamous cell carcinoma through IL-6/STAT3 signaling pathway. J Cancer. 2020 Feb 19;11(9):2621-2631.  [Content Brief]