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Ataxin-3 Protein, Human (His)

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Based on 1 Customer Validation

Ataxin-3 is a deubiquitinating enzyme that maintains proteostasis, regulates transcription, and is involved in cytoskeletal dynamics, myogenesis, and degradation of misfolded chaperone substrates. It trims long polyubiquitin chains and interacts with STUB1/CHIP to limit ubiquitin chain length, preventing further elongation. Ataxin-3 Protein, Human (His) is the recombinant human-derived Ataxin-3 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Ataxin-3 Protein, Human (His)
  • Experimental Validation Results for Ataxin-3 Protein, Human (His)
  • Ataxin-3 Protein, Human (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Ataxin-3 is a deubiquitinating enzyme that maintains proteostasis, regulates transcription, and is involved in cytoskeletal dynamics, myogenesis, and degradation of misfolded chaperone substrates. It trims long polyubiquitin chains and interacts with STUB1/CHIP to limit ubiquitin chain length, preventing further elongation. Ataxin-3 Protein, Human (His) is the recombinant human-derived Ataxin-3 protein, expressed by E. coli , with N-6*His labeled tag.

Background

Ataxin-3 protein, a deubiquitinating enzyme, plays a crucial role in maintaining protein homeostasis, regulating transcription, cytoskeleton dynamics, myogenesis, and participating in the degradation of misfolded chaperone substrates. Demonstrating an affinity for long polyubiquitin chains, Ataxin-3 trims them, particularly focusing on chains with more ubiquitin moieties, while exhibiting weak or no activity against shorter chains. It engages in the degradation of misfolded chaperone substrates by interacting with STUB1/CHIP, where it restricts the length of ubiquitin chains attached to STUB1/CHIP substrates, preventing further chain extension. Additionally, Ataxin-3 acts as a histone-binding protein, influencing transcription regulation, and serves as a negative regulator of mTORC1 signaling during amino acid deprivation by mediating the deubiquitination of RHEB. Furthermore, Ataxin-3 contributes to autophagy regulation by deubiquitinating 'Lys-402' of BECN1, leading to BECN1 stabilization.

Verified Bioactivity

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 this effect is 2.979 ng/ml, corresponding to a specific activity is 3.36×105 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 2.979 ng/mL, corresponding to a specific activity is 3.36×105 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    P54252-2 (M1-K361)

  • Gene ID
    4287  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • Ataxin-3 (M1-K361)
      Accession # P54252-2
    • C-term
  • Protein Length

    Full Length of Isoform-2

  • Synonyms

    ATXN3; MJD1; Prev. SCA3; Olivopontocerebellar Ataxia 3; Prev. MJD; Ubiquitinyl Hydrolase 1; ATX3; Ataxin 3 Variant An; JOS; Ataxin 3 Variant H; Spinocerebellar Ataxia Type 3 Protein; Ataxin 3 Variant E; Machado-Joseph Disease Protein 1; Josephin; Ataxin-3

  • AA Sequence

    MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKK

  • Molecular Weight

    Approximately 43 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 2.979 ng/mL, corresponding to a specific activity is 3.36×105 units/mg.

  • Ataxin-3 Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for Ataxin-3 Protein, Human (His)
  • Experimental Validation Results for Ataxin-3 Protein, Human (His)
  • Ataxin-3 Protein, Human (His) Product Image