B7-2/CD86 Protein, Rabbit (HEK293, His)
Based on 1 Customer Validation
The B7-2/CD86 protein is a key receptor that provides costimulatory signals critical for T lymphocyte proliferation and interleukin-2 production through CD28 or CTLA-4 binding. It plays a decisive role in early T cell activation, affecting immunity or anergy within 24 hours. B7-2/CD86 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived B7-2/CD86 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Rabbit
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The B7-2/CD86 protein is a key receptor that provides costimulatory signals critical for T lymphocyte proliferation and interleukin-2 production through CD28 or CTLA-4 binding. It plays a decisive role in early T cell activation, affecting immunity or anergy within 24 hours. B7-2/CD86 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived B7-2/CD86 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
B7-2/CD86 Protein functions as a receptor pivotal for the costimulatory signal necessary for T-lymphocyte proliferation and interleukin-2 production, achieved by binding to CD28 or CTLA-4. It plays a crucial role in the early events of T-cell activation and costimulation of naive T-cells, contributing to the decision between immunity and anergy within 24 hours after activation. Additionally, B7-2/CD86 participates in the regulation of B cell function, influencing the level of IgG(1) produced. Upon CD40 engagement, it activates the NF-kappa-B signaling pathway through phospholipase C and protein kinase C activation. The protein forms homodimers and interacts with MARCH8. Furthermore, it engages with PHB1 and PHB2, with the interactions intensifying after priming with CD40.
Technical Parameters
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Species Rabbit
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Source HEK293
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Tag C-6*His
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Accession
P42071 (A23-R246)
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Molecular Construction
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N-term
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B7-2 (A23-R246)
Accession # P42071 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CD86; FUN-1; Prev. CD28LG2; B70; T-Lymphocyte Activation Antigen CD86; B-Lymphocyte Activation Antigen B7-2; B7.2; B-Lymphocyte Antigen B7-2; B7-2; CD86 Antigen; CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen); CD86 V6; CTLA-4 Counter-Receptor B7.2; LA
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AA Sequence
AASLRIQAYFNKTADLPCQFTNSQSRSLSELVVFWQDQERLVLYELFLGREKPDNVDPKYIGRTSFDQESWNLQLHNVQIKDKGVYQCFVHHRGAKGLVPIYQMNSELSVLANFTQPEITLISNITRNSAINLTCSSVQGYPEPKKMFFVLKTENATTEYDGVIEKSQDNVTGLYNISISGSITFSDDIRNATIYCVLQTESTETYSQHFPIVPADPVPVEKPR
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Predicted Molecular Mass
26.3 kDa
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Molecular Weight
Approximately 38-70 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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