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BAI2 Protein, Human (HEK293, hFc)

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Based on 1 Customer Validation

The BAI2 protein is an orphan G protein-coupled receptor involved in cell adhesion and possible cell-cell interactions. It activates the NFAT signaling pathway through the G protein GNAZ and contributes to transcriptional regulation. BAI2 Protein, Human (HEK293, hFc) is the recombinant human-derived BAI2 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for BAI2 Protein, Human (HEK293, hFc)
  • Experimental Validation Results for BAI2 Protein, Human (HEK293, hFc)
  • BAI2 Protein, Human (HEK293, hFc) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The BAI2 protein is an orphan G protein-coupled receptor involved in cell adhesion and possible cell-cell interactions. It activates the NFAT signaling pathway through the G protein GNAZ and contributes to transcriptional regulation. BAI2 Protein, Human (HEK293, hFc) is the recombinant human-derived BAI2 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

The BAI2 Protein, an orphan G-protein coupled receptor, plays a significant role in cell adhesion and likely participates in cell-cell interactions. By activating the NFAT-signaling pathway through the G-protein GNAZ, BAI2 contributes to transcriptional regulation, highlighting its involvement in diverse cellular processes. Additionally, BAI2 is implicated in angiogenesis inhibition. Notably, the receptor activity of BAI2 is subject to regulation through proteolytic processing. The long N-terminal region exerts an inhibitory effect on constitutive signaling activity, and removal of this region induces an increase in receptor activity. These intricate regulatory mechanisms underscore the versatility of BAI2 in modulating cellular responses and provide insights into its potential as a key player in cellular communication and angiogenic processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 2 μg/mL can bind Biotinylated Recombinant Human BAI2. The ED50 for this effect is 82.33 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    O60241-3 (F21-D911)

  • Gene ID
    576  [NCBI]
  • Molecular Construction
    • N-term
    • BAI2 (F21-D911)
      Accession # O60241-3
    • hFc
    • C-term
  • Protein Length

    Partial

  • Synonyms

    ADGRB2; Prev. BAI2; Adhesion G Protein-Coupled Receptor B2; Brain-Specific Angiogenesis Inhibitor 2

  • AA Sequence

    FDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKD

  • Predicted Molecular Mass

    123.7 kDa

  • Molecular Weight

    Approximately 128-150 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 2 μg/mL can bind Biotinylated Recombinant Human BAI2. The ED50 for this effect is 82.33 ng/mL.

  • BAI2 Protein, Human (HEK293, hFc) Product Image
    1/16
  • Experimental Validation Results for BAI2 Protein, Human (HEK293, hFc)
  • Experimental Validation Results for BAI2 Protein, Human (HEK293, hFc)
  • BAI2 Protein, Human (HEK293, hFc) Product Image