BAI3 Protein, Human (HEK293, His)

BAI3 Protein, Human (HEK293, His) is a recombinant human brain-specific angiogenesis inhibitor 3 produced in HEK293 cells. Brain-specific angiogenesis inhibitor 3 (BAI3) is a member of the cell-adhesion class of G protein-coupled receptors that are expressed at high levels in the brain. BAI3 is a high-affinity receptor for C1q-like proteins^[1].

BAI3 is from the adhesion group of seven-transmembranespanning G protein-coupled receptors (GPCRs) and has been identified via gene expression profiling asbeing upregulated in small-cell lung cancer (SCLC) tumors. It has subsequently been validated as a sen-sitive and specific immunohistochemical marker for SCLC, helping to differentiate these tumors frommorphologically similar large-cell neuroendocrine (LCNEC) malignancies^[2].

Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 1 µg/mL (100 µL/well) can bind Human BAI3. The ED₅₀ for this effect is 0.7891 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 1 μg/mL (100 μL/well) can bind Human BAI3. The ED₅₀ for this effect is 0.7891 μg/mL.

Human

HEK293

C-6*His

O60242 (A25-T880)

577  [NCBI]

Extracellular Domain

AQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVFLQYDKNFIQIRRVFPTNFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLILLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRIRTCQGAVITGQQCEGTGEEVRRCNEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRASYIPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNLDLILPTLRNYTVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVT

Approximately 90-145 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% thehalose, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Bolliger MF, et al. The cell-adhesion G protein-coupled receptor BAI3 is a high-affinity receptor for C1q-like proteins. Proc Natl Acad Sci U S A. 2011;108(6):2534-2539.  [Content Brief]

  [2]. Thomas M, et al. An investigation into the potential role of brain angiogenesis inhibitor protein 3 (BAI3) in the tumorigenesis of small-cell carcinoma: a review of the surrounding literature. J Recept Signal Transduct Res. 2017;37(4):325-334.  [Content Brief]