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BAI3 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

BAI3 Protein, Human (HEK293, His) is a recombinant human brain-specific angiogenesis inhibitor 3 produced in HEK293 cells. Brain-specific angiogenesis inhibitor 3 (BAI3) is a member of the cell-adhesion class of G protein-coupled receptors that are expressed at high levels in the brain. BAI3 is a high-affinity receptor for C1q-like proteins.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for BAI3 Protein, Human (HEK293, His)
  • Experimental Validation Results for BAI3 Protein, Human (HEK293, His)
  • BAI3 Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

BAI3 Protein, Human (HEK293, His) is a recombinant human brain-specific angiogenesis inhibitor 3 produced in HEK293 cells. Brain-specific angiogenesis inhibitor 3 (BAI3) is a member of the cell-adhesion class of G protein-coupled receptors that are expressed at high levels in the brain. BAI3 is a high-affinity receptor for C1q-like proteins[1].

Background

BAI3 is from the adhesion group of seven-transmembranespanning G protein-coupled receptors (GPCRs) and has been identified via gene expression profiling asbeing upregulated in small-cell lung cancer (SCLC) tumors. It has subsequently been validated as a sen-sitive and specific immunohistochemical marker for SCLC, helping to differentiate these tumors frommorphologically similar large-cell neuroendocrine (LCNEC) malignancies[2].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 1 µg/mL (100 µL/well) can bind Human BAI3. The ED50 for this effect is 0.7891 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 1 μg/mL (100 μL/well) can bind Human BAI3. The ED50 for this effect is 0.7891 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    O60242 (A25-T880)

  • Gene ID
    577  [NCBI]
  • Molecular Construction
    • N-term
    • BAI3 (A25-T880)
      Accession # O60242
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    ADGRB3; Brain-Specific Angiogenesis Inhibitor 3; Prev. BAI3; DJ91B17.1 (Brain-Specific Angiogenesis Inhibitor 3); Adhesion G Protein-Coupled Receptor B3; KIAA0550

  • AA Sequence

    AQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVFLQYDKNFIQIRRVFPTNFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLILLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRIRTCQGAVITGQQCEGTGEEVRRCNEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRASYIPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNLDLILPTLRNYTVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVT

  • Molecular Weight

    Approximately 115-145 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% thehalose, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Bolliger MF, et al. The cell-adhesion G protein-coupled receptor BAI3 is a high-affinity receptor for C1q-like proteins. Proc Natl Acad Sci U S A. 2011;108(6):2534-2539. [Content Brief]

[2]. Thomas M, et al. An investigation into the potential role of brain angiogenesis inhibitor protein 3 (BAI3) in the tumorigenesis of small-cell carcinoma: a review of the surrounding literature. J Recept Signal Transduct Res. 2017;37(4):325-334. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 1 μg/mL (100 μL/well) can bind Human BAI3. The ED50 for this effect is 0.7891 μg/mL.

  • BAI3 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for BAI3 Protein, Human (HEK293, His)
  • Experimental Validation Results for BAI3 Protein, Human (HEK293, His)
  • BAI3 Protein, Human (HEK293, His) Product Image