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BAI3 Protein, Human (HEK293, His)

Cat. No.: HY-P7675
COA Handling Instructions

BAI3 Protein, Human (HEK293, His) is a recombinant human brain-specific angiogenesis inhibitor 3 produced in HEK293 cells. Brain-specific angiogenesis inhibitor 3 (BAI3) is a member of the cell-adhesion class of G protein-coupled receptors that are expressed at high levels in the brain. BAI3 is a high-affinity receptor for C1q-like proteins.

For research use only. We do not sell to patients.

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Description

BAI3 Protein, Human (HEK293, His) is a recombinant human brain-specific angiogenesis inhibitor 3 produced in HEK293 cells. Brain-specific angiogenesis inhibitor 3 (BAI3) is a member of the cell-adhesion class of G protein-coupled receptors that are expressed at high levels in the brain. BAI3 is a high-affinity receptor for C1q-like proteins[1].

Background

BAI3 is from the adhesion group of seven-transmembranespanning G protein-coupled receptors (GPCRs) and has been identified via gene expression profiling asbeing upregulated in small-cell lung cancer (SCLC) tumors. It has subsequently been validated as a sen-sitive and specific immunohistochemical marker for SCLC, helping to differentiate these tumors frommorphologically similar large-cell neuroendocrine (LCNEC) malignancies[2].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

O60242 (A25-T880)

Gene ID

577  [NCBI]

Molecular Construction
N-term
BAI3 (A25-T880)
Accession # O60242
6*His
C-term
Synonyms
rHuBAI3, His; Brain-Specific Angiogenesis Inhibitor 3; BAI3
AA Sequence

AQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVFLQYDKNFIQIRRVFPTNFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQSLILLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGTQQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRIRTCQGAVITGQQCEGTGEEVRRCNEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRASYIPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTGNVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNLDLILPTLRNYTVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCKTVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTHHHHHH

Molecular Weight

115-145 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, 5% Thehalose, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

BAI3 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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