BCAM Protein, Mouse (HEK293, His)

BCAM Protein, acting as a laminin alpha-5 receptor, potentially participates in intracellular signaling, indicated by its resemblance to related proteins. BCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived BCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of BCAM Protein, Mouse (HEK293, His) is 516 a.a., with molecular weight of 70-90 kDa.

The BCAM protein functions as a laminin alpha-5 receptor and may play a role in mediating intracellular signaling, as suggested by its similarities with related proteins.

Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When 5x10⁴ cells per well are added to Recombinant Mouse BCAM coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀ for this effect is 0.491 μg/mL. Corresponding to a specific activity is 2.036×10³ U/mg

• 
  Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When 5 x 10⁴ cells per well are added to Recombinant Mouse BCAM coated plates, cell adhesion is enhanced in a dose dependent manner. The ED⁵⁰ for this effect is 0.491 μg/mL, corresponding to a specific activity is 2.036×10³ U/mg

Mouse

HEK293

C-His

Q9R069 (E26-A541)

57278  [NCBI]

ELHVSVPPRVEVMRGEQVALDCTPREHPEHYVLEWFLVDGTGARHRLASVEPQGSEFLGTVHSLGRVPPYEVDSRGRLVIAKVQVGDGRDYVCVVKAGAAGTSEATSSVRVFATPEDTEVSPNKGTLSVMDQFAQEIATCSSNNGNPVPRITWYRNGQRLEVPMEVNQKGYITIRTVREASGLYSLTSTLYLRLHKDDRDANFHCAAHYDLPSGQHGRLDSHTFRLTLHYPTEHVEFWVGSPSTTEGWVREGDAVQLLCQGDGSPSPEYSFFRQQGTQEEQLNVNLKGNLTLERVHRNQSGIYGCRVEDYDADEEVQLVKKLKLHVAYLDPLELSVPEELFVFLNSSSTVVNCSARGLPTPTVRWTKDSVTLADGPMLSLQSVTFDSAGTYTCEASTPTVPLLSRTQSFQLIVQGAPELKPNEIMPKSGNSWTEGDEVMLTCSARGFPEPKLTWSQRGDTPAEPPFEGRGWKSSSLMVKVTSALSREGVSCEASNIHGKKGHVFHFGSVAPQTAQA

70-90 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.