BCAM Protein, Rat (HEK293, His)
Based on 1 Customer Validation
Nectin-2/CD112 Protein, functioning as a laminin alpha-5 receptor, displays potential involvement in intracellular signaling, reflecting its similarity to related proteins. BCAM Protein, Rat (HEK293, His) is the recombinant rat-derived BCAM protein, expressed by HEK293 , with C-His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
Nectin-2/CD112 Protein, functioning as a laminin alpha-5 receptor, displays potential involvement in intracellular signaling, reflecting its similarity to related proteins. BCAM Protein, Rat (HEK293, His) is the recombinant rat-derived BCAM protein, expressed by HEK293 , with C-His labeled tag.
Background
The Nectin-2/CD112 protein serves as a laminin alpha-5 receptor and exhibits potential for mediating intracellular signaling, as inferred by its similarity to related proteins.
Verified Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells.When cells are added to coated plates(10 μg/mL,100 μL/well), approximately 20-50% will adhere after 1 hour at 37°C.
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-His
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Accession
Q9ESS6 (E26-A543)
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Molecular Construction
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N-term
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BCAM (E26-A543)
Accession # Q9ESS6 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
BCAM; F8/G253 Antigen; Prev. LU; Lutheran Blood Group (Auberger B Antigen Included); B-CAM Cell Surface Glycoprotein; Basal Cell Adhesion Molecule Lutheran Blood Group; Basal Cell Adhesion Molecule; Lutheran Blood Group Variant LUGA; F8/G253; Lutheran Blo
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AA Sequence
ELHVSVPPRVEVMRGEQIALDCTPREHPENYVLEWLLVDASGARHRLASVEPQGSEFLGTIHNSRGRRPPYKIDSLGRLVIAEAQVGDERDYVCVVKAGAAGTSEATSSVRVFATPEATEVAPNKGTLSVMEQFAQEIATCSSNNGNPVPRITWYQNGQRLDVPMELNSKGYMTSRTVREASGLYSLTSTLYLRLHKEDRDASFHCAAHYDLPSGQHGRLDSHTFRLTLHYPTEHVEFWVGSPSTTEGWVREGDAVQLLCQGDGSPSPEYSFFREQGNQEEQLNVNLKGNLTLEGVHRSQSGIYGCRVEDYDADEEVQLVKKLKLHVAYLDPLELSVPEEFSVFLNSSGTVVNCSARGLPAPIVRWTKDSVTVADGPILSLDSVTFDSAGTYTCEASTPTVPLLSRTQSFQLVVQGAPELKPNEIKPKSGTSWTEGDEVMLTCSARGFPEPKLTWSQRGDTTPAEPPFEGRGWMSSSLTLKVTSALSREGVSCEASNIHGKNGHVFHFGSVAPQTAQA
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Predicted Molecular Mass
58.2 kDa
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Molecular Weight
Approximately 68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)