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PRCP Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

PRCP protein acts as an enzyme that cleaves the C-terminal amino acid linked to proline in peptides, including angiotensin II, III and des-Arg9-bradykinin. Although some substrates retain enzymatic activity even at neutral pH, this cleavage activity is noteworthy because it occurs at acidic pH. PRCP Protein, Human (HEK293, His) is the recombinant human-derived PRCP protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for PRCP Protein, Human (HEK293, His)
  • PRCP Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

PRCP protein acts as an enzyme that cleaves the C-terminal amino acid linked to proline in peptides, including angiotensin II, III and des-Arg9-bradykinin. Although some substrates retain enzymatic activity even at neutral pH, this cleavage activity is noteworthy because it occurs at acidic pH. PRCP Protein, Human (HEK293, His) is the recombinant human-derived PRCP protein, expressed by HEK293 , with C-6*His labeled tag.

Background

PRCP, or prolylcarboxypeptidase, is an enzyme known for its role in selectively cleaving C-terminal amino acids linked to proline in peptides like angiotensin II, III, and des-Arg9-bradykinin. A distinctive feature of PRCP is its preference for acidic pH during cleavage; however, it retains enzymatic activity with specific substrates even under neutral pH conditions. This dual pH responsiveness implies a versatile functionality for PRCP in different cellular environments, influencing the processing and bioactivity of peptides involved in essential physiological pathways, particularly those associated with the renin-angiotensin and kinin systems. The ability of PRCP to operate across a range of pH levels suggests its intricate regulatory role in peptide metabolism and signaling.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P42785 (L22-H496)

  • Gene ID
    5547  [NCBI]
  • Molecular Construction
    • N-term
    • PRCP (L22-H496)
      Accession # P42785
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PRCP; Angiotensinase C; Prolylcarboxypeptidase; HUMPCP; PCP; Lysosomal Carboxypeptidase C; Lysosomal Pro-X Carboxypeptidase; CDNA FLJ33528 Fis, Clone BRAMY2007110, Highly Similar To Lysosomal Pro-X Carboxypeptidase; Proline Carboxypeptidase; Prolylcarboxy

  • AA Sequence

    LRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH

  • Predicted Molecular Mass

    54.5 kDa

  • Molecular Weight

    Approximately 60-90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM NaAc-HAc, 150 mM NaCl, 10% glycerol, pH4.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • PRCP Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PRCP Protein, Human (HEK293, His)
  • PRCP Protein, Human (HEK293, His) Product Image