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C-Reactive Protein, Human (His)

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C-Reactive Protein, an acute-phase protein, is a marker of inflammation and is produced by the liver. It is used as a diagnostic tool to assess the presence and severity of inflammation in various diseases. C-Reactive Protein's role in inflammation detection and its clinical significance in disease management make it a subject of interest in medical research. C-Reactive Protein, Human (His) is the recombinant human-derived C-Reactive protein, expressed by E. coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for C-Reactive Protein, Human (His)
  • Experimental Validation Results for C-Reactive Protein, Human (His)
  • C-Reactive Protein, Human (His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

C-Reactive Protein, an acute-phase protein, is a marker of inflammation and is produced by the liver. It is used as a diagnostic tool to assess the presence and severity of inflammation in various diseases. C-Reactive Protein's role in inflammation detection and its clinical significance in disease management make it a subject of interest in medical research. C-Reactive Protein, Human (His) is the recombinant human-derived C-Reactive protein, expressed by E. coli , with C-6*His labeled tag.

Background

C-Reactive Protein (CRP) exhibits various functions related to host defense. It facilitates agglutination, bacterial capsular swelling, phagocytosis, and complement fixation by binding to phosphorylcholine in a calcium-dependent manner. CRP is also capable of interacting with DNA and histones, and it may scavenge nuclear material that is released from damaged circulating cells.

Verified Bioactivity

1.Immobilized Human CRP-His at 10 μg/mL (100 μl/well) can bind Human CD32a-His Biotinylated by NHS-biotin prior to testing.The ED50 is 7.64 μg/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human CD32a at 2 μg/mL (100 μL/well) can bind Biotinylated Human C-Reactive. The ED50 for this effect is 1-5 μg/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    P02741-1 (Q19-P224)

  • Gene ID
    1401  [NCBI]
  • Molecular Construction
    • N-term
    • C-Reactive (Q19-P224)
      Accession # P02741-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    CRP; C-Reactive Protein, Pentraxin-Related; C-Reactive Protein; Pentraxin 1; PTX1

  • AA Sequence

    QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP

  • Molecular Weight

    Approximately 27 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 0.1% SKL.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human CD32a at 2 μg/mL (100 μL/well) can bind Biotinylated Human C-Reactive. The ED50 for this effect is 1.182 μg/mL.

  • C-Reactive Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for C-Reactive Protein, Human (His)
  • Experimental Validation Results for C-Reactive Protein, Human (His)
  • C-Reactive Protein, Human (His) Product Image