C2/Complement C2 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Complement C2 is a component of the classical pathway of the complement system and is cleaved by activated factor C1, resulting in the formation of two distinct fragments: C2b and C2a. The subsequent serine protease activity of C2a is critical for its subsequent interaction with the complement factor C4b, leading to the formation of C3 or C5 convertase. C2/Complement C2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived C2/Complement C2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Complement C2 is a component of the classical pathway of the complement system and is cleaved by activated factor C1, resulting in the formation of two distinct fragments: C2b and C2a. The subsequent serine protease activity of C2a is critical for its subsequent interaction with the complement factor C4b, leading to the formation of C3 or C5 convertase. C2/Complement C2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived C2/Complement C2 protein, expressed by HEK293 , with C-His labeled tag.
Complement C2, a vital component of the classical pathway within the complement system, undergoes cleavage by activated factor C1 into two fragments: C2b and C2a. The generated C2a fragment, possessing serine protease activity, subsequently associates with complement factor C4b, culminating in the formation of the C3 or C5 convertase. This enzymatic complex plays a pivotal role in the activation cascade of the complement system, facilitating the downstream proteolytic cleavage of complement factors C3 and C5, which are integral steps in the immune response. The orchestrated interplay of C2 in the classical pathway underscores its significance in mediating immune defense mechanisms and maintaining immune homeostasis. (
Measured by its ability to cleave a colorimetric peptide 200 μM substrate Z-GR-SBzl, in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 129.61 pmol/min/μg.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
P21180-1 (A19-L760)
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Molecular Construction
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N-term
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C2 (M1-L760)
Accession # P21180 -
His
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C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
Complement C2; C3/C5 convertase; C2
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AA Sequence
ALFCNQNVNITGGNFTLSHGWAPGSLLIYSCPLGRYPSPAWRKCQSNGQWLTPRSSSHHTLRSSRMVKAVCKPVRCLAPSSFENGIYFPRLVSYPVGSNVSFECEQDFTLRGSPVRYCRPNGLWDGETAVCDNGASHCPNPGISVGTARTGLNFDLGDKVRYRCSSSNMVLTGSAERECQSNGVWSGSEPICRQPYSYDFPEDVASALDTSLTNLLGATNPTQNLLTKSLGRKIIIQRSGHLNLYLLLDASQSVTEKDFDIFKKSAELMVERIFSFEVNVSVAIITFASQPKTIMSILSERSQDVTEVITSLDSASYKDHENATGTNTYEVLIRVYSMMQSQMDRLGMETSAWKEIRHTIILLTDGKSNMGDSPKKAVTRIRELLSIEQNRDDYLDIYAIGVGKLDVDWKELNELGSKKDGERHAFILQDAKALQQIFEHMLDVSKLTDTICGVGNMSANASDQERTPWQVTFKPKSKETCQGSLISDQWVLTAAHCFHDIQMEDHHLWRVNVGDPTSQHGKEFLVEDVIIAPGFNVHAKRKQGISEFYADDIALLKLSRKVKMSTHARPICLPCTVGANMALRRSPGSTCKDHETELLSQQKVPAHFVALNGNRLNINLRTGPEWTRCIQAVSQNKNIFPSLTNVSEVVTDQFLCSGMEEEDDNPCKGESGGAVFLGRRYRFFQVGLVSWGLFDPCHGSSNKNLRKKPPRGVLPRDFHISLFRLQPWLRQHLDGVLDFLPL
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Molecular Weight
Approximately 91 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
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≥ 95%, as determined by reducing SDS-PAGE.
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Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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