CACNG1 Protein, Human (Cell-Free, His)
CACNG1 is a key component of the L-VGCC γ subunit and interacts with Gal3. CACNG1 Protein, Human (Cell-Free, His) is a recombinant human CACNG1 protein expressed in E. coli cell-free and tagged with N-10*His.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
CACNG1 is a key component of the L-VGCC γ subunit and interacts with Gal3. CACNG1 Protein, Human (Cell-Free, His) is a recombinant human CACNG1 protein expressed in E. coli cell-free and tagged with N-10*His.
Background
CACNG1, a key component of the γ subunits of L-VGCCs, interacted with Gal3. Galectin-3 (Gal3) acutely reduces glucose-stimulated insulin secretion (GSIS) in β-cell lines and primary islets in mice and humans. Importantly, Gal3 binds to calcium voltage-gated channel auxiliary subunit gamma 1 (CACNG1) and inhibits calcium influx via the cytomembrane and subsequent glucose-stimulated insulin secretion (GSIS)[1].
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-10*His
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Accession
Q06432 (M1-H222)
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Gene ID786
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Molecular Construction
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N-term
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10*His
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CACNG1 (M1-H222)
Accession # Q06432 -
C-term
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Protein Length
Full Length
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Synonyms
CACNG1; Calcium Channel, Voltage-Dependent, Gamma Subunit 1; Prev. CACNLG; Neuronal Dihydropyridine-Sensitive Calcium Channel Gamma Subunit; Dihydropyridine-Sensitive L-Type, Skeletal Muscle Calcium Channel Subunit Gamma; L-Type Calcium Channel Gamma Poly
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AA Sequence
MSQTKMLKVRVTLFCILAGIVLAMTAVVTDHWAVLSPHMEHHNTTCEAAHFGLWRICTKRIPMDDSKTCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAIAIFSLGFIILGSLCVLLSLGKKRDYLLRPASMFYAFAGLCILVSVEVMRQSVKRMIDSEDTVWIEYYYSWSFACACAAFILLFLGGLALLLFSLPRMPRNPWESCMDAEPEH
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Predicted Molecular Mass
31 kDa
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Molecular Weight
Approximately 31 kDa & 66 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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